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条斑紫菜壳孢子超微结构研究
引用本文:周文君,李?? 赟,戴继勋.条斑紫菜壳孢子超微结构研究[J].海洋科学,2007,31(2):1-4.
作者姓名:周文君  李?? 赟  戴继勋
作者单位:中国海洋大学,海水养殖教育部重点实验室,山东,青岛,266003
基金项目:国家高技术研究发展计划(863计划)
摘    要:应用戊二醛、锇酸固定,环氧树脂包埋,醋酸双氧铀-柠檬酸铅制作切片,以透射电镜观察条斑紫菜(Porphyra yezoensis)壳孢子的超微结构,结果表明:壳孢子外包被一层薄的细胞壁,中央为一个由同心片层结构的类囊体膜构成的轴生星状色素体,色素体中有一个无淀粉鞘包被的蛋白核,类囊体膜上附有嗜锇性的质体小球.壳孢子细胞核位于色素体的一侧,呈不规则的长圆形,核仁偏于核的一侧.在胞质中分布有线粒体、红藻淀粉、液泡等细胞器.与放散前的成熟壳孢子囊枝细胞超微结构比较,成熟壳孢子超微结构的变化主要有细胞壁变薄,色素体结构松散甚至退化,蛋白核分裂,液泡数量增多而红藻淀粉数量减少等,这些变化反映了成熟壳孢子基本细胞特征,同时反映了在壳孢子生长发育过程中温度下降、光照时间缩短和光照强度减弱等环境因素的变化.

关 键 词:条斑紫菜(Porphyra  yezoensis)  壳孢子  超微结构  条斑紫菜  壳孢子  微结构研究  Porphyra  yezoensis  conchospore  ultrastructure  环境因素  光照强度  时间缩短  温度下降  发育过程  孢子生长  细胞特征  核分裂  蛋白  退化  变薄  变化  结构比较  孢子囊枝
文章编号:1000-3096(2007)02-0001-04
收稿时间:2005-06-28
修稿时间:2005-11-10

Study on ultrastructure of conchospore of Porphyra yezoensis
ZHOU Wen-jun,LI Yun,DAI Ji-xun.Study on ultrastructure of conchospore of Porphyra yezoensis[J].Marine Sciences,2007,31(2):1-4.
Authors:ZHOU Wen-jun  LI Yun  DAI Ji-xun
Institution:Key Laboratory, of Mariculture, Education Ministry of China, Ocean University of China, Qingdao 266003, China
Abstract:With observation of transmission electron microscope, ultrastructure of conchospore of Porphyra yezoensis was studied, it was shown that thin cell wall appeared outside of conchospore, while in the center of it, a star-shaped chromatophore thylakoid existed. There is no starch sheath pyrenoid embedded in chromatophore. Lipid bodies stained with osmium could be observed on the membrane of thylakoid. A round- like nucleus located in the chromatophore with deeper darkness. There are other cellular organs observed such as floridean starch, mitochondria, vacuole and so on. Besides, this study indicated that mature conchosporangial cell phase was important for conchospore release and influenced by environmental factors like temperature, length and intensity of sunlight.
Keywords:Porphyra yezoensis  conchospore  ultrastrueture
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