| 海洋放线菌H2003代谢产物中环二肽成分及其抗肿瘤活性的初步研究 |
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| 引用本文： | 李 冬,朱伟明,顾谦群,崔承彬,朱天骄,刘红兵,方玉春.海洋放线菌H2003代谢产物中环二肽成分及其抗肿瘤活性的初步研究[J].海洋科学,2007,31(5):45-48. |
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| 作者姓名： | 李 冬 朱伟明 顾谦群 崔承彬 朱天骄 刘红兵 方玉春 |
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| 作者单位： | 1. 教育部海洋药物重点实验室,山东省海洋药物重点实验室,中国海洋大学,海洋药物与食品研究所,山东,青岛,266003
2. 教育部海洋药物重点实验室,山东省海洋药物重点实验室,中国海洋大学,海洋药物与食品研究所,山东,青岛,266003;军事医学科学院,毒物药物研究所,北京,100850 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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山东省科技攻关项目
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山东省科学院科研项目
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教育部长江学者奖励计划
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山东省青岛市自然科学基金 |
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| 摘 要： | 海洋来源放线菌H2003发酵产物的粗提物对小鼠乳腺癌细胞tsFT210具有明显的细胞毒活性。为了发现其抗肿瘤活性成分,采用活性跟踪的分离方法,经多次硅胶柱色谱、凝胶柱色谱及高效液相色谱分离,从菌株H2003发酵液中分离得到了7个环二肽化合物。经核磁共振光谱、质谱和旋光分析,其结构分别鉴定为cyclo(L-Leu-L-Pro)(1),cyclo(D-Val-L-Pro)(2),cyclo(Gly-L-Leu)(3),cyclo(L-Pro-L-Try)(4),cyclo(Gly-D-Phe)(5),cyclo(Gly-L-Pro)(6)和cyclo(L-Ala-L-Phe)(7)。其中化合物1通过SRB法测试表现出弱的抗肿瘤活性,系首次报道。
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| 关 键 词： | 海洋放线菌 次级代谢产物 环二肽 抗肿瘤活性 |
| 文章编号： | 1000-3096（2007）05-0045-04 |
| 收稿时间： | 2004-09-20 |
| 修稿时间： | 2004-12-10 |
Structural identification and anti-tumor activity of Diketopi-perazines from secondary metabolites of marine-derived actinomycete H2003 |
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| LI Dong,ZHU Wei-ming,GU Qian-qun,CUI Cheng-bin,ZHU Tian-jiao,LIU Hong-bing,FANG Yu-chun.Structural identification and anti-tumor activity of Diketopi-perazines from secondary metabolites of marine-derived actinomycete H2003[J].Marine Sciences,2007,31(5):45-48. |
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| Authors: | LI Dong ZHU Wei-ming GU Qian-qun CUI Cheng-bin ZHU Tian-jiao LIU Hong-bing FANG Yu-chun |
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| Abstract: | The fermentation of the marine-derived actinomycete H2003 showed toxicity on tsFT210 cells. In order to isolate and identify the bioactive components, the secondary metabolite of the fermentation of H2003 was studied. Through bioassay-guided fractionation of necrosis on tsFT210 cells, seven diketopiperazines were isolated from the secondary metabolites of actinomycete H2003 by repeating chromatography over silica gel, sephadex LH-20 and HPLC. Their structures were identified by the spectroscopic methods and anti-tumor activities were assayed by SRB method. Compound(1) showed weak anti-tumor activity on tsFT210 cells. |
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| Keywords: | marine-derived actinomycete secondary metabolite diketopiperazine anti-tumor activity |
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