| 裙带菜配子体总RNA提取的比较研究 |
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| 引用本文： | 李世国,佟少明,侯和胜.裙带菜配子体总RNA提取的比较研究[J].海洋科学,2011,35(4):21-25. |
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| 作者姓名： | 李世国 佟少明 侯和胜 |
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| 作者单位： | 辽宁师范大学,生命科学学院,辽宁省植物生物技术重点实验室,辽宁,大连,116029 |
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| 基金项目： | 中国科学院实验海洋生物学重点实验室开放基金项目 |
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| 摘 要： | 以裙带菜(Undaria pinnatifida)配子体为材料对比分析了4种方法,改进SDS法、CTAB法,Trizol试剂盒、RNAiso Reagent试剂盒法提取RNA的质量和纯度,并采用RT-PCR对mRNA反转录,对mRNA的可用性进行了检测.结果表明,两种试剂盒Trizol和RNAiso Reagent及其...
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| 关 键 词： | 裙带菜(Undaria pinnatifida) 配子体 RNA提取 RT-PCR |
| 收稿时间： | 2010/1/10 0:00:00 |
| 修稿时间： | 2010/4/12 0:00:00 |
Comparison of methods for total RNA extraction from Undaria pinnatifida gametophyte |
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| LI Shi-guo,TONG Shao-ming,HOU He-sheng.Comparison of methods for total RNA extraction from Undaria pinnatifida gametophyte[J].Marine Sciences,2011,35(4):21-25. |
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| Authors: | LI Shi-guo TONG Shao-ming HOU He-sheng |
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| Institution: | LI Shi-guo,TONG Shao-ming,HOU He-sheng(College of life sciences,Liaoning Normal University,Liaoning Provincial key laboratory of Plant Biotech-nology,Dalian 116029,China) |
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| Abstract: | In this paper, modified SDS method, CTAB method, and Trizol and RNAiso Reagent kits were applied to extract total RNA from Undaria pinnatifida gametophyte. We compared the quality and purity of the extracted RNAs and used RT-PCR to examine whether the RNAs were functionally active. The results showed that the Trizol and RNAiso Reagent kits were not suitable to seaweed RNA isolation, because there was no clear band detected on the gel. The RNA isolated by the CTAB method showed smear bands and also was contaminated by genomic DNA. However, the modified SDS method could give birth to good quality RNAs, although there was a little genomic DNA contamination. The OD260/OD280 ratio of the RNA was between 1.8 and 2.0. These RNAs could be used for RT-PCR. So the modified SDS method is a very good method for extracting total RNAs from gametophyte of U. pinnatifida and some other seaweeds. |
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| Keywords: | Undaria pinnatifida gametophyte RNA extraction RT-PCR |
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