| 海洋生物蛋白的酶解及酶解产物的抗氧化活性 |
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| 引用本文： | 吴继卫,何海伦,路敬涛,陈秀兰,张玉忠.海洋生物蛋白的酶解及酶解产物的抗氧化活性[J].海洋科学,2005,29(3):76-80. |
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| 作者姓名： | 吴继卫 何海伦 路敬涛 陈秀兰 张玉忠 |
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| 作者单位： | 山东大学,微生物技术国家重点实验室,山东,济南,250100 |
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| 基金项目： | 国家海洋863计划资助项目(2003AA625040) |
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| 摘 要： | 应用4种蛋白酶：实验室保藏菌株Bacillus sp．SM98011和Bacillus sp．SM97010所产的中性蛋白酶SM98011和SM97010以及购自诺维信公司的碱性蛋白酶和风味蛋白酶分别酶解鲨鱼、牡蛎、扇贝和毛虾，分析了酶解液中蛋白质含量、氨基酸组成及肽的含量，并测定了酶解液的抗氧化能力。结果表明，酶解液中必需氨基酸含量丰富、均衡，营养价值较高；SM98011蛋白酶、SM97010蛋白酶和碱性蛋白酶的酶解液中肽的比例较高；而风味蛋白酶的酶解产物主要以游离的风味氨基酸为主，肽的含量较低。SM97010和SM98011蛋白酶的酶解液具有较高的抗氧化能力，预示在功能食品开发方面具有较好的前景。
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| 关 键 词： | 游离氨基酸 肽 蛋白酶 抗氧化性 |
| 文章编号： | 1000-3096(2005)03-0076-05 |
| 收稿时间： | 2004/11/9 0:00:00 |
| 修稿时间： | 2004年11月9日 |
Enzymatic hydrolysis of marine proteins and hydrolysates antioxidant activity |
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| WU Ji-wei,HE Hai-lun,LU Jing-Tao,CHEN Xiu-lan,ZHANG Yu-zhong.Enzymatic hydrolysis of marine proteins and hydrolysates antioxidant activity[J].Marine Sciences,2005,29(3):76-80. |
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| Authors: | WU Ji-wei HE Hai-lun LU Jing-Tao CHEN Xiu-lan ZHANG Yu-zhong |
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| Abstract: | Marine proteins, shark, oyster,scallop, shrimp were hydrolyzed with four proteases: SM98011 produced by Bacillus sp.; SM98011, SM97010 produced by Bacillus sp. SM97010; Alcalase, and Flavourzyme. Their content of protein, amino acids and peptides were tested. All of the hydrolysates were rich in essential amino acids. The hydrolysates of marine proteins digested with protease SM98011, SM97010 and Alcalase were enriched in peptides. Compared to other three proteases, the Flavourzyme can dissociate more flavor amino acids. Antioxidant activity of each hydrolysate was determined. The hydrolysates from protease SM98011 and protease SM97010 possess more notable antioxidant activity than other two protease hydrolysates. |
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| Keywords: | free amino acid peptides protease antioxidant |
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