| 气升式光生物反应器培养裙带菜配子体的初步研究 |
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| 引用本文： | 张栩,李大鹏,蔡昭铃,丛威,欧阳藩.气升式光生物反应器培养裙带菜配子体的初步研究[J].海洋科学,2002,26(6):39-43. |
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| 作者姓名： | 张栩 李大鹏 蔡昭铃 丛威 欧阳藩 |
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| 作者单位： | 1. 中国科学院过程工程研究所生化工程国家重点实验室,北京,100080
2. 中国科学院海洋研究所,青岛,266071 |
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| 基金项目： | 生化工程国家重点实验室科学基金课题KT2000 02号 |
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| 摘 要： | 在升气式光生物反应器中培养裙带菜配子体无性系。比生长速率最高为0.262d^－1，在快速生长期，日平均增重达到30％。通过在线测定DO和pH值变化实时了解配子体光合、呼吸作用的情况，通过测定碱度分析 子体对无机碳的利用情况，表明HCO3一直处于较高浓度，为配子体所利用的主要碳源。测定培养液的盐度、NO3^-和PO4^3-的浓度，其中NO3^-的利用量与配子体的生长相对应，而盐度变化可反映配子体对营养盐的作用。
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| 关 键 词： | 气升式光生物反应器 培养 裙带菜 配子体 无性繁殖 育苗 |
| 收稿时间： | 2001/11/13 0:00:00 |
| 修稿时间： | 2001年11月13 |
STUDIES ON GROWTH OF GAMETOPHYTES OF Undaria pinnatifida IN AIRLIFT PHOTOBIOREACTOR |
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| ZHANG Xu LI Da,peng CAI Zhao,ling CONG Wei OUYANG Fan.STUDIES ON GROWTH OF GAMETOPHYTES OF Undaria pinnatifida IN AIRLIFT PHOTOBIOREACTOR[J].Marine Sciences,2002,26(6):39-43. |
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| Authors: | ZHANG Xu LI Da peng CAI Zhao ling CONG Wei OUYANG Fan |
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| Institution: | ZHANG Xu1 LI Da peng2 CAI Zhao ling1 CONG Wei1 OUYANG Fan1 |
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| Abstract: | The cultures of gametophytes clone of Undaria pinnatifida were carried out in an airlift photobioreactor. The highest rate of specific growth was 0.262 d 1 and daily average rate of liveweight growth was 30% in fast growing period. By on line monitoring DO and pH in cultivation process, the capability of photosynthesis and respiration of gametophytes were found out in real time. Alkalinity of culture solution was assayed and inorganic carbon consumption of gametophytes was inferred. The results indicated free CO2 in the cultivation system was first utilized by gametophytes and the concentration was not very high in the cultivation process. Meanwhile, was compensated through respiration of gametophytes in a certain degree and the higher concentration of was maintained in the cultivation.The salinity and concentration of and of culture solution were analyzed. The results indicated consumption of was corresponding to the growth of gametophytes and the variation of salinity reflected the utilization of nutritive salt of gametophytes. |
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| Keywords: | Undaria pinnatifida Gametophytes Photobioreactor |
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