| 用包埋脱水法冰冻保存牟氏角刺藻 |
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| 引用本文： | 李莹,王起华,李贺,李婷婷.用包埋脱水法冰冻保存牟氏角刺藻[J].海洋科学,2003,27(7):48-51. |
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| 作者姓名： | 李莹 王起华 李贺 李婷婷 |
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| 作者单位： | 辽宁师范大学生命科学学院,大连,116029 |
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| 基金项目： | 国家自然科学基金项目30170099号;辽宁省教育厅科研基金项目20041015号. |
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| 摘 要： | 采用包埋脱水法研究了牟氏角刺藻(Chaetaceros muelleri)的超低温保存，探讨了影响存活率的内、外因素：藻细胞年龄、蔗糖浓度、脱水速率、胶球含水量等。结果 表明，将处于静止初期的藻细胞包埋在含有0．5mol／L蔗糖的3％褐藻酸钙胶球中，以平均速率0．9％含水量／h脱水至胶球含水量为40％的条件下冰冻存活率较高，可达到30％左右。与常规的两步法冰冻保存相比。包埋脱水法可大大简化操作程序，是保存牟氏角刺藻的一种有潜力的方法。
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| 关 键 词： | 包埋脱水法 冰冻保存 牟氏角刺藻 操作程序 |
| 文章编号： | 1000-3096(2003)07-0048-04 |
| 收稿时间： | 2002/10/8 0:00:00 |
| 修稿时间： | 2002年10月8日 |
CRYOPRESERVATION OF Chaetoceros muelleri BY ENCAPSULATION-DEHYDRATION |
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| LI Ying WANG Qi-Hua LI He LI Ting-Ting.CRYOPRESERVATION OF Chaetoceros muelleri BY ENCAPSULATION-DEHYDRATION[J].Marine Sciences,2003,27(7):48-51. |
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| Authors: | LI Ying WANG Qi-Hua LI He LI Ting-Ting |
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| Abstract: | Chaetoceros muelleri was preserved in liquid nitrogen by encapsulation-dehydration. The main factors influencing the algal viabilitiy after cryopreservation, such as the age of algal cells, the sucrose concentration, the dehydration rate and the water content of beads, were studied.The results showed that the algal viability after cryopreservation reached about 30%-a relatively high viable rate when the algal cells in a stationary phase were encapsulated in the beads of 3% Ca-alginate with 0. 5 mol/L sucrose and the beads were dehydrated at an average rate of 0. 9% water content/h to 40% of the water content. Compared with the conventional two-step freezing method, the encapsulation-dehydration method simplified the operation procedure of cryopreservation greatly and so was a potential method for preservation of Chaetoceros muelleri . |
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| Keywords: | Chaetoceros muelleri Encapsulation-dehydration Cryopreservation |
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