| 脂多糖对栉孔扇贝血清和血细胞中7种酶活力的影响 |
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| 引用本文： | 孙虎山,李光友.脂多糖对栉孔扇贝血清和血细胞中7种酶活力的影响[J].海洋科学,1999,23(4):54-58. |
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| 作者姓名： | 孙虎山 李光友 |
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| 作者单位： | 1. 烟台师范学院,264025
2. 国家海洋局第一海洋研究所,青岛,266003 |
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| 基金项目： | 国家攀登计划B资助项目! PDB 6－6－3号 |
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| 摘 要： | 实验于１９９８年１￣１０月进行。栉孔扇贝注射脂多糖后，分别于１，１５和３０ｈ测定了其血清和血细胞中７种参与免疫防御的水解酶、氧化酶和抗氧化酶的活力。结果表明，血清实验组的酸性磷酸酶（ＡＣＰ）活力在１，１５和３０ｈ时，血细胞实验组在１ｈ时显著高于对照组。血清和血细胞实验组的碱性磷酸酶（ＡＫＰ）活力仅在１ｈ时，溶菌酶活力分别在１ｈ和１５ｈ时显著高于对照组。血细胞中无酚氧化酶（ＰＯ）活性。血清实验组的Ｐ
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| 关 键 词： | 脂多糖 栉孔扇贝 血清 血细胞 酶活力 |
| 收稿时间： | 1999/4/26 0:00:00 |
| 修稿时间： | 5/6/1999 12:00:00 AM |
EFFECTS OF LIPOPOLYSACCHARIDE ON ENZYMES IN SERUM AND HAEMOCYTES OF Chlamys farreri |
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| SUN Hu-shan,LI Guang-you.EFFECTS OF LIPOPOLYSACCHARIDE ON ENZYMES IN SERUM AND HAEMOCYTES OF Chlamys farreri[J].Marine Sciences,1999,23(4):54-58. |
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| Authors: | SUN Hu-shan LI Guang-you |
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| Abstract: | Seven kinds of hydrolytic, oxidant and antioxidant enzymes participating in the immune defence in the haemolymph of Chlamys farreri were assayed at 1, 15 and 30 h after injection with lipopolysaccharide (LPS) from January to Octo- ber, 1998. The results were as follows: The ACP activities of experimental groups in serum or haemocytes were much higher than that of control groups at 1, 15 and 30 h or at I h. The AKP activities of experimental groups in serum and haemocytes were much higher than control group only at 1 h. The lysozyme activities of experimental groups in serum or haemocytes were much higher than control groups at 1 h or at 15 h. There were no activities of PO in haemocytes. The PO or MPO activities of experimental groups in serum were much higher than control groups at 1 h or at 15 h. The SOD or CAT activities of experimental groups in serum and haernocytes were much higher than control group at 1 h or at 1, 15 and 30 h. It was suggested that LPS could stimulated and enhance the activities of lysozyme, ACP, AKP, PO, MPO, SOD and CAT. |
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| Keywords: | Lipopolysaccharide Chlamys farreri Serum Haemocytes Activity of enzyme |
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