| 海藻提取物环氧加酶-2 抑制活性研究 |
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| 引用本文： | 史大永,李晓红,李敬,郭书举,苏华.海藻提取物环氧加酶-2 抑制活性研究[J].海洋科学,2009,33(7):30-32. |
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| 作者姓名： | 史大永 李晓红 李敬 郭书举 苏华 |
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| 作者单位： | 1. 中国科学院,海洋研究所,山东,青岛,266071
2. 青岛市市立医院,山东,青岛,266011 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，中国科学院方向性创新项目 |
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| 摘 要： | 系统采集中国沿海10种代表性海藻,进行有效成分提取与粗分;利用昆虫杆状病毒表达系统克隆人COX-2基因,并在昆虫Spodoptera frugiperda(sf9)细胞中表达获得COX-2蛋白;以花生四烯酸为底物,通过测定前列腺素PGE2的生成浓度,测定海藻不同部位对COX-2酶的抑制活性.结果表明,萱藻(Scytosiphon lomentarius)乙醇提取物、鼠尾藻(Sargassum thunbergii)和刺松藻(Codium fragile)乙酸乙酯相、松节藻(Rhodomela conferroides)正丁醇相表现出良好的COX-2酶抑制活性(质量浓度为10μg/mL时,抑制率大于50%).首次对上述海藻进行COX-2酶抑制活性研究,其中萱藻乙醇提取物、鼠尾藻和刺松藻乙酸乙酯相、松节藻正丁醇相表现出良好的COX-2抑制活性.
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| 关 键 词： | 海藻 环氧加酶-2(COX-2) 抑制活性 |
| 收稿时间： | 2008/11/26 0:00:00 |
| 修稿时间： | 2009/1/20 0:00:00 |
Inhibitory activity against cyclooxygenase-2 enzyme of extraction from algae |
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| SHI Da-yong,LI Xiao-hong,LI Jing,GUO Shu-ju and SU Hua.Inhibitory activity against cyclooxygenase-2 enzyme of extraction from algae[J].Marine Sciences,2009,33(7):30-32. |
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| Authors: | SHI Da-yong LI Xiao-hong LI Jing GUO Shu-ju and SU Hua |
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| Abstract: | Cyclooxygenase-2 (COX-2) is a key enzyme that catalyzes the biosynthesis of prostaglandins from arachidonic acid and plays a critical role in inflammation, pain and fever. In order to search for new type inhibitors against COX-2, extractions from 10 algae were screened indirectly by determination of prostaglandin E2 which was synthesized from arachidonic acid. Human cyclooxygenase-2 genes were cloned from human monocyte cell line THP-1 cells and expressed in Spodoptera frugiperda (sf9) insect cell line by Bac-to-Bac baculovirus expression systems. The results showed that ethanolic extraction of Scytosiphon lomentarius, EtOAc phase of Sargassum thunbergii and Codium fragile and butanolic phase of Rhodomela confervoides exhibited good bioactivities with inhibitory ratio higher than 50% at a dose of 10 μg/mL. |
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| Keywords: | algae cyclooxygenase-2 (COX-2) inhibitory activity |
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