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中国对虾淋巴器官中球形病毒的电镜观察
引用本文:汝少国,姜明,李永祺,贾翠红,丛日山,王悦.中国对虾淋巴器官中球形病毒的电镜观察[J].海洋科学,1999,23(5):44-48.
作者姓名:汝少国  姜明  李永祺  贾翠红  丛日山  王悦
作者单位:青岛海洋大学,266003
基金项目:国家攀登计划B资助项目!PDB6-7-1号
摘    要:应用光镜和电镜对中国对虾淋巴器官的显微结构和超微结构进行观察,结果表明,淋巴器官的盲管主要由内皮细胞和基质细胞构成,细胞间隙内充满着大量的以颗粒细胞为主的血细胞,

关 键 词:中国对虾  淋巴器官  显微结构  超微结构  球形病毒
收稿时间:1998/8/20 0:00:00
修稿时间:1998-08-20

THE ELECTRON MICROSCOPE EXAMINATON OF SPHERICAL VIRUS IN LYMPHOID ORGAN CELLS OF Penaeus chinensis
RU Shao-guo,JIANG Ming,LI Yong-qi,JIA Cui-hong,CONG Ri-shan,WANG Yue.THE ELECTRON MICROSCOPE EXAMINATON OF SPHERICAL VIRUS IN LYMPHOID ORGAN CELLS OF Penaeus chinensis[J].Marine Sciences,1999,23(5):44-48.
Authors:RU Shao-guo  JIANG Ming  LI Yong-qi  JIA Cui-hong  CONG Ri-shan  WANG Yue
Abstract:With the use of the optical microscope and the electron microscope, the results through observing the histological structure and ultrastructure of lymphoid organ from Penaeus chinensis reveal that under the optical microscope, the lym- phoid organ consists of many occluded anastomosing tubules. between two tubules are lumens, which are composed of the endothelial cells and the stromal cells. The interstitial sinuses are abundant in lots of hemocytes. And under the electron microscope, the non-nuclear parts of the endothelial cells have processes toward the lumens. The stromal cells have a large number of Golgi apparatus and mitochondria, whose structure are very simple. Moreover, we found a spherical virus in lymphoid organ cells which has an envelope and a diameter of 70-100 nm. The morphological properties of this virus is similar to REO(Reoviridae). Most inclusion bodies are globose and there are no capsulae in outer layer. Infected by the virus, the cellular organs showed serious cytopathological changes, such as the partial dilation, disruption or even complete disappearance of the nuclear membrane, the oedema of the Golgi apparatuses and the endoplastic reticulum, and the disintegration or denaturation of the mitochondria cristae. Simultaneously, together with the virus, some my coplasma infected the cells too.
Keywords:Penaeus chinensis  Lymphoid organ  Histological structure  Ultrastructure  Spherical virus
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