| 长牡蛎细胞周期调控关键基因cyclin B3 的克隆及其在性腺发育中的作用 |
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| 引用本文： | 王 桐,李 莉,阙华勇,张国范.长牡蛎细胞周期调控关键基因cyclin B3 的克隆及其在性腺发育中的作用[J].海洋科学,2011,35(12):1-9. |
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| 作者姓名： | 王 桐 李 莉 阙华勇 张国范 |
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| 作者单位： | 中国科学院 海洋研究所；中国科学院 研究生院;中国科学院 海洋研究所;中国科学院 海洋研究所;中国科学院 海洋研究所 |
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| 基金项目： | 国家“863”计划资助项目(2006AA10A401); 国家“973”计划资助项目(2010CB126401); 国家公益性行业(农业)科研专项(3-53) |
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| 摘 要： | 首次在长牡蛎（Crassostrea gigas）中克隆得到细胞周期蛋白B3（cyclin B3）的cDNA 全长序列和基因组结构序列。cyclin B3 基因cDNA 全长2383 bp, 其中编码区长度为1293 bp, 编码一条含430 个氨基酸的多肽链, 氨基酸序列比对和结构域分析均表明其为其他物种cyclin...
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| 关 键 词： | 长牡蛎(Crassostrea gigas) 细胞周期调控 cyclin B3 性腺 Real Time PCR |
| 收稿时间： | 2010/12/25 0:00:00 |
| 修稿时间： | 2011/2/25 0:00:00 |
Molecular cloning and characterization of the key regulator of cell cycle cyclin B3 in Pacific Oyster (Crassostrea gigas), and its role in gonad development |
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| Abstract: | The full length cDNA sequence of cyclin B3 was cloned from Pacific oyster (Crassostrea gigas) for the first time. Cyclin B3 cDNA was 2383 bp in length, containing a 1293bp CDS that encoded a peptide of 430 amino acids with a calculated molecular weight of 47.8 kD. Multiple alignment and converted domain analysis showed that this peptide was a homolog of cyclin B3. The gDNA sequence of cyclin B3 contained 10 exons and 9 introns, which was consistent with the position in evolution of C. gigas. Expression of cyclin B3 gene was found in all tissues, but also showed a very strong tissue-specific feature; the amount of cyclin B3 in gonad was the highest among tissues, 100 times of mantle, the lowest expression tissue. The mRNA amount of cyclin B3 in gonad increased with the maturation of gonad. The expression implied the important role of cyclin B3 in mitosis, meiosis and early development of embryo. |
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| Keywords: | Pacific oyster (Crassostrea gigas) cell cycle regulation cyclin B3 gonad Real Time PCR |
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