| 大黄鱼野生群体与养殖群体遗传多样性研究 |
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| 引用本文： | 陈淑吟,徐士霞,张志勇,郭忠仁,孙中响,朱立静.大黄鱼野生群体与养殖群体遗传多样性研究[J].海洋科学,2011,35(12):82-87. |
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| 作者姓名： | 陈淑吟 徐士霞 张志勇 郭忠仁 孙中响 朱立静 |
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| 作者单位： | 江苏省海洋水产研究所;南京师范大学 生命科学院 动物遗传资源研究所;江苏省海洋水产研究所;江苏省海洋水产研究所;江苏省海洋水产研究所；苏州大学 医学部基础医学与生物科学学院;江苏省海洋水产研究所；苏州大学 医学部基础医学与生物科学学院 |
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| 基金项目： | “十一五”国家科技支撑计划重大项目(2006BAD09A01) |
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| 摘 要： | 利用线粒体COI 基因序列片段与ISSR 标记方法, 研究江苏海域大黄鱼（Pseudosciaena crocea）野生群体（JS）的遗传多样性水平, 并比较其与浙江（ZJ）、福建（FJ）养殖群体的遗传差异。结果表明： （1）扩增COI 基因序列片段625bp, 3 个群体65 个序列中有15 个单倍型, 群体间有3 ...
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| 关 键 词： | 大黄鱼(Pseudosciaena crocea) COI ISSR 遗传多样性 群体遗传差异 |
| 收稿时间： | 2010/8/11 0:00:00 |
| 修稿时间： | 2/6/2011 12:00:00 AM |
Study of genetic diversity of wild and culture populations of Pseudosciaena crocea using two molecular markers |
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| Abstract: | Large yellow croaker, Pseudosciaena crocea Richardson 1846, is a commercially important marine fish and now is very scarce. The fish could be found only occasionally in the Lvsu Fisheries Region in Jiangsu(JS) province, and no researches were carried out about this wild population. In the present study, partial mitochondrial cytochrome oxidase I gene (COI) gene sequence and inter-simple sequence repeat (ISSR) markers were used to investigate the genetic diversity of this wild population and two cultivated populations from Zhejiang(ZJ) and Fujian(FJ) province. The results were as follows: (1) The amplified fragment was 625 bp, revealing 15 haplotypes and three haplotypes of which were shared by these populations. The JS wild population has nine unique haplotypes and two haplotypes shared with the ZJ population. Nei's coefficient of differentiation(Gst) between populations were -0.0196 (JS - ZJ), 0.02115(JS- FJ) and -0.0125(FJ-ZJ), respectively; (2) Ten informative and reliable primers were selected to investigate the genetic diversity. The proportation of polymorphic loci of JS, ZJ and FJ populations were 74.59%, 63.93% and 59.02%, respectively. Nei's genetic identity between populations were 0.9246(JS-ZJ), 0.8310(JS-FJ) and 0.8826(FJ-ZJ), respectively. |
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| Keywords: | Pseudosciaena crocea COI partial sequence ISSR genetic diversity population genetic differentiation |
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