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免疫增强剂对鲫鱼非特异性免疫功能的影响
引用本文:刘云,孙峰,王丹.免疫增强剂对鲫鱼非特异性免疫功能的影响[J].海洋科学,2004,28(9):42-45.
作者姓名:刘云  孙峰  王丹
作者单位:中国海洋大学,海洋生命学院,山东,青岛,266003
基金项目:国家自然科学基金资助项目 (30000129),国家重点基础研究发展规划973资助项目 (G1999012005)
摘    要:利用主要成分为从:每洋甲壳类中提取的甲壳胺作为免疫增强剂,在饵料中添加不同的浓度(0.5%,1%,2%),在0,10,20,30d时检测鲫鱼(Carassius auratus)的白细胞数量、白细胞吞噬功能的变化以及血清中酚氧化酶活力等免疫指标的变化。研究结果表明,该免疫增强剂可以提高鲫鱼血液中白细胞数量和吞噬细胞的吞噬能力,增强鲫鱼血清中酚氧化酶的活力.对鲫鱼的生长无明显影响。鲫鱼的非特异性免疫功能随着免疫增强剂浓度的增加和投喂时间的延长而呈现增强的趋势。

关 键 词:鲫鱼(Carassius  auratus)  免疫增强剂  甲壳胺  白细胞数量  吞噬功能  酚氧化酶活力
文章编号:1000-3096(2004)09-0042-04
收稿时间:2003/6/25 0:00:00
修稿时间:2003年6月25日

Effects of immunostimulants on the non- specific immunity of crucian (Carassius auratus)
LIU Yun,SUN Feng,WANG Dan.Effects of immunostimulants on the non- specific immunity of crucian (Carassius auratus)[J].Marine Sciences,2004,28(9):42-45.
Authors:LIU Yun  SUN Feng  WANG Dan
Abstract:To determine the effects of chitosan on innate immune response of crucian (Carassius auratus), fish were fed diets containing 0 (control), 0.5%,1%,2% chitosan for 10, 20 and 30 days. The growth rate, the number of leucocytes and the phagocytes functions together with the phenol oxidase activities were studied at each of the assayed time. The results showed that the number of leucocytes increased and the phagocytic activities were enhanced with the phenol oxidase activities were greatly increased, at the same time, no statistically significant impact on the growth rate was observed. These results showed the effects of immunostimulants on non-specific immunity of crucian enhanced with increased dose and time.
Keywords:crucian  (Carassius auratus)  immunostimulants  chitosan  leucocytes  phagocytosis  phenol oxidase activities
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