| 双水相萃取法富集分离螺旋藻藻蓝蛋白的研究 |
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| 引用本文： | 刘 杨,王雪青,庞广昌,谢卓峰.双水相萃取法富集分离螺旋藻藻蓝蛋白的研究[J].海洋科学,2008,32(7):30-32. |
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| 作者姓名： | 刘 杨 王雪青 庞广昌 谢卓峰 |
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| 作者单位： | 天津市食品生物技术重点实验室,天津商业大学,生物技术与食品科学学院,天津300134 |
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| 摘 要： | 以PEG／硫酸钠双水相体系，经一次萃取从钝顶螺旋藻（Spirulina platensis）细胞破碎液中富集分离藻蓝蛋白。结果表明，萃取最适宜的条件为12％PEG4000，15％Na2SO4，1％KCl，藻蓝蛋白收率为91．2％，分配系数达到8．01，分离因数达到6．33。对于螺旋藻藻蓝蛋白的富集分离，双水相萃取法与传统的盐析沉淀法相比，具有节省操作时间、简化操作过程、降低能耗和成本以及易于工艺放大等优点。
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| 关 键 词： | 螺旋藻（Spirulina） 藻蓝蛋白 聚乙二醇 双水相体系 萃取 |
| 收稿时间： | 8/1/2007 12:00:00 AM |
| 修稿时间： | 2008/4/10 0:00:00 |
Aqueous two-phase extraction for enrichment and isolation of c-phycocyanin from Spirulina |
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| LIU Yang,WANG Xue-qing,PANG Guang-chang,Xie Zhuo-feng.Aqueous two-phase extraction for enrichment and isolation of c-phycocyanin from Spirulina[J].Marine Sciences,2008,32(7):30-32. |
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| Authors: | LIU Yang WANG Xue-qing PANG Guang-chang Xie Zhuo-feng |
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| Institution: | LIU Yang,WANG Xue-qing,PANG Guang-chang,Xie Zhuo-feng(Tianjin Key Laboratory of Food Biotechnology(PEG),College of Biotechnology , Food Science,Tianjin University of Commerce,Tianjin 300134,China) |
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| Abstract: | Aqueous two-phase system composed of PEG/ Na2SO4 was once to enrich and isolate c-phycocyanin from the supernatant of disrupted Spirulina cell in this paper.The optimum extraction conditions were: 12% PEG,15% Na2SO4,1% KCl,the partition coefficient of c-phycocyanin was as high as 8.01,with a yield of 91.2%.The separation factor was increased to 6.33.The preliminary study indicated that aqueous two-phase extraction was better than traditional salt-out precipitation for enrichment and isolation of c-phycocyan... |
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| Keywords: | Spirulina c-phycocyanin polyethylene glycol(PEG) aqueous two-phase system extraction |
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