| 许氏平鲉微卫星标记与体长、体质量、体高的相关性分析 |
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| 引用本文： | 韩承慧,马海涛,薛 蕊,姜海滨,常 城,王 腾.许氏平鲉微卫星标记与体长、体质量、体高的相关性分析[J].海洋科学,2016,40(12):47-55. |
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| 作者姓名： | 韩承慧 马海涛 薛 蕊 姜海滨 常 城 王 腾 |
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| 作者单位： | 山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室；上海海洋大学水产与生命学院,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室,中国科学院 海洋研究所,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室；上海海洋大学水产与生命学院,山东省海洋资源与环境研究院 山东省海洋生态修复重点实验室；上海海洋大学水产与生命学院 |
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| 基金项目： | 山东省良种工程项目(2014-2017) |
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| 摘 要： | 为了解许氏平鲉(Sebastes schlegelii)微卫星标记与生长性状的相关性,选取26个微卫星标记在海捕许氏平鲉群体中进行微卫星筛选,每个微卫星位点的等位基因数(Na)在3~21,有效等位基因数(Ne)为1.1815~15.5676;观测杂合度(Ho)范围为0.0417~0.9999;期望杂合度(He)范围为0.1581~0.9456;多态信息含量(PIC)范围为0.1509~0.9320。利用单标记分析法分析了多态性较高(PIC0.5)的22个标记与体长、体质量、体高的相关性,结果显示3个标记与生长性状显著相关,其中HJ2-32与体长、体质量和体高显著相关(P0.05),并推断等位基因D与C对于体质量、体长、体高分别具有重要的正向与负向影响;HJ7-2与体质量显著相关;HJ7-22与体长显著相关,并推测等位基因F对体长具有正向影响,等位基因B对体长有负面影响。这些标记可为许氏平鲉分子标记辅助育种提供基础。
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| 关 键 词： | 许氏平鲉(Sebastes schlegelii) 微卫星标记 生长性状 相关性分析 |
| 收稿时间： | 2015/10/30 0:00:00 |
| 修稿时间： | 2015/12/23 0:00:00 |
Correlation analysis of microsatellite markers with body length, weight, and height in Sebastes schlegelii |
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| HAN Cheng-hui,MA Hai-tao,XUE Rui,JIANG Hai-bin,CHANG Cheng and WANG Teng.Correlation analysis of microsatellite markers with body length, weight, and height in Sebastes schlegelii[J].Marine Sciences,2016,40(12):47-55. |
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| Authors: | HAN Cheng-hui MA Hai-tao XUE Rui JIANG Hai-bin CHANG Cheng and WANG Teng |
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| Abstract: | Twenty-six polymorphic microsatellite markers were selected to estimate a wild population of Sebastes schlegelii for studying the correlation between microsatellite markers and growth traits, which revealed that the number of alleles was 260 and the allele numbers ranged from 3 to 21 per locus. The observed and expected heterozygosity varied from 0.0417 to 0.9999 and from 0.1581 to 0.9456, respectively. The values of polymorphic information content ranged from 0.1509 to 0.9320. Twenty-two microsatellite loci with high polymorphism were used to analyze the correlation with body length, weight, and height by single marker-based analysis. The results showed that these markers significantly correlated with growth traits; loci HJ2-32 significantly correlated with three growth traits, and alleles D and C had positive and negative effects on growth traits, respectively. HJ7-2 showed a significant correlation with body weight; alleles F and B of HJ7-22 had positive and negative effects on length traits, respectively. These aforementioned markers would be of benefit for a further study on molecular marker-assisted breeding of S. schlegelii. |
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| Keywords: | Sebastes schlegelii microsatellite growth traits correlation analysis |
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