| T-E染色法用于对虾暴发性流行病的现场快速诊断 |
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| 引用本文： | 黄倢,杨丛海,于佳,宋晓玲.T-E染色法用于对虾暴发性流行病的现场快速诊断[J].海洋科学,1995,19(1):29-34. |
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| 作者姓名： | 黄倢 杨丛海 于佳 宋晓玲 |
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| 作者单位： | 中国水产科学研究院黄海水产研究所!青岛266003 |
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| 摘 要： | 用Ｔ－Ｅ染色法可对该病进行现场快速诊断。取发病对虾的胃上皮区组织 或鳃组织，用Ｔ－Ｅ染液进行压片染色，可观察到上皮细胞核和结缔组织细胞核的病 毒性病理变化：细胞核质被挤到核的边沿，核内空泡化。发病严重的对虾胞核显著 膨大，核内呈嗜伊红着色。用这一染色法在现场仅需１０ｍｉｎ就可掌握对虾的细胞病 理变化，诊断结果与组织切片的Ｈ－Ｅ染色法一致。
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| 关 键 词： | T-E染色法 对虾暴发性流行病 快速诊断 |
| 收稿时间： | 1994/9/24 0:00:00 |
RAPID ON-SITE DIAGNOSIS OF THE EXPLOSIVE EPIDERMIC DISEASE OF SHRIMP (SEEDS) BY T-E STAINING |
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| Huang Jie,Yang Conghai,Yu Jia and Song Xiaoling.RAPID ON-SITE DIAGNOSIS OF THE EXPLOSIVE EPIDERMIC DISEASE OF SHRIMP (SEEDS) BY T-E STAINING[J].Marine Sciences,1995,19(1):29-34. |
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| Authors: | Huang Jie Yang Conghai Yu Jia and Song Xiaoling |
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| Abstract: | The shrimp explosive epidermic disease (EEDS) was resulted from the baculoviral hepodermal and hematopoietic necrosis. T-E (Trypan blue--Eosin Y) staining was used for the rapid on--site diagnosis of EEDS. The viral pathological changes of intranuclear vacuolation were observed in the epithelial and connective tissue of the stomach or gill of diseased shrimp in the squashes stained with T-E stain. The cell nucleus of seriously diseased shrimp became inflated and eosinophile. T-E staining had the same diagnosis results with the method of histological H-E staining of about 10 minutes. |
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| Keywords: | T-E staining The shrimp explosive epidermic disease Rapid on--site diagnosis |
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