| 溶藻弧菌的间接荧光抗体快速检测 |
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| 引用本文： | 王军,鄢庆枇,苏永全,张纹.溶藻弧菌的间接荧光抗体快速检测[J].海洋科学,2002,26(7):1-4. |
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| 作者姓名： | 王军 鄢庆枇 苏永全 张纹 |
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| 作者单位： | 1. 厦门大学海洋学系,厦门大学亚热带海洋研究所,361005
2. 厦门大学海洋学系,厦门大学亚热带海洋研究所,361005;集美大学水产生物技术研究所、水产学院厦门,361021 |
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| 基金项目： | 国家高技术研究发展计划资助项目863 819 02012号 |
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| 摘 要： | 建立了大黄鱼病原溶藻弧菌的间接荧光抗体检测方法，溶藻弧菌的特异性抗血清由新西兰兔制备，试管凝集法测定抗血清及交叉反应效价，吸附离心法去除交叉反应，荧光抗体为异硫氰酸荧光素标记的羊抗兔免疫球蛋白，现场检测对具典型弧菌病表症大黄鱼病原检出率为100％，表观健康大黄鱼病原检出率为30％，养殖海水检测结果均为阴性，结果表明，间接荧光抗体检测法不仅可以用于已感染发病大黄鱼的快速检测，而且还能用于已感染未发病大黄鱼的检测。
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| 关 键 词： | 溶藻弧菌 间接荧光抗体 快速检测 大黄鱼 病原菌 |
| 收稿时间： | 2001/12/31 0:00:00 |
| 修稿时间： | 2001年12月31 |
STUDIES ON THE RAPID DETECTION OF Vibrio alginolyticus BY INDIRECT FLUORESCENT ANTIBODY STAINING |
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| WANG Jun YAN Qing,pi SU Yong,quan ZHANG Wen.STUDIES ON THE RAPID DETECTION OF Vibrio alginolyticus BY INDIRECT FLUORESCENT ANTIBODY STAINING[J].Marine Sciences,2002,26(7):1-4. |
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| Authors: | WANG Jun YAN Qing pi SU Yong quan ZHANG Wen |
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| Institution: | WANG Jun1 YAN Qing pi2 SU Yong quan1 ZHANG Wen1 |
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| Abstract: | A rapid detection of Vibrio alginolyticus infecting the Large yellow croaker Pseudosciaena crocea by indirect fluorescent antibody staining has been developed. The specific antisera for V. alginolyticus was prepared with New Zealand rabbit,and cross reactive efficacy was tested by coagulation in tube. It seemed that the goat anti rabbit IgG had been marked by fluorescent isothiocyanate (FITC). The results showed that the positive reaction detected in situ from the typical symptom P. crocea infected by Vibrio was 100%,30% from the health ones also suffered by this pathogen , but negative detection from its aquaculture sea water. It demonstrated that the rapid detection by indirect fluorescent antibody could be not only used for testing the ill yellow croaker infected by pathogen Vibrio, but also for those healthy ones with little pathogen. |
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| Keywords: | Pseudosciaena crocea Vibrio alginolyticus Fluorescent antibody Rapid detection |
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