| 对虾鳗弧菌卵黄抗体(IgY)的制备及其对人工感染的保护研究 |
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| 引用本文： | 朱香萍,张再生,刘宗柱.对虾鳗弧菌卵黄抗体(IgY)的制备及其对人工感染的保护研究[J].海洋科学,2008,32(2):25-28. |
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| 作者姓名： | 朱香萍 张再生 刘宗柱 |
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| 作者单位： | 1. 青岛农业大学,山东,青岛266109
2. 烟台出入境检验检疫局,蓬莱办事处,山东,蓬莱,265600 |
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| 基金项目： | 山东省出入境检验检疫局资助项目 |
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| 摘 要： | 以纯培养的鳗弧菌作为抗原,探讨对虾鳗弧菌(Vibrio anguillarum)卵黄抗体(IgY)的制备技术,以及IgY在对虾体内的代谢及对人工感染和自然感染弧菌病的保护作用。结果表明:鳗弧菌对蛋鸡有较好的免疫原性,产蛋鸡经免疫后,血清与卵黄中很快出现抗体,而且卵黄中的抗体水平要高于血清中的抗体水平。IgY对鳗弧菌的感染有可靠的保护作用,IgY经口服后很快到达直肠,继而血淋巴、肝胰脏中也出现抗体,并可持续12~24 h;投喂IgY的试验组对虾在7 d内的死亡率为15%,极显著地低于对照组对虾的同期死亡率(为60%)。
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| 关 键 词： | 对虾 弧菌病 被动免疫 卵黄抗体(IgY) |
| 文章编号： | 1000-3096（2008）02-0025-04 |
| 收稿时间： | 2007-07-19 |
| 修稿时间： | 2007-12-12 |
Preparation of shrimp Vibrio anguillarum IgY and its effect on protecting from artificial infection |
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| ZHU Xiang-ping,ZHANG Zai-sheng,LIU Zong-zhu.Preparation of shrimp Vibrio anguillarum IgY and its effect on protecting from artificial infection[J].Marine Sciences,2008,32(2):25-28. |
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| Authors: | ZHU Xiang-ping ZHANG Zai-sheng LIU Zong-zhu |
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| Abstract: | The purly cultivated Vibrio anguillarum was taken as an antigen to explore the preparation technology and the metabolism in shrimp body of IgY,and the effect on protecting from artificially infected Vibrio anguillarum.The result showed that Vibrio anguillarum had a strong immunity of laying hens.Antibody was detected in blood serum and vitellus soon after the laying hens were immunized,and the antibody-level in vitellus was higher than that in serum.After oral administration,IgY could be detected at rectum,bloodlymph and also in hepaticopancrea of shrimp which lasts about 12~24 h.The death rate within 7 days of the tested group was 15%,significantly lower than that of the control(60%,P<0.01), showing good protection of IgY from vibrion infection. |
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| Keywords: | shrimp Vibrion passive immunity IgY(egg yolk immunoglobulin) |
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