| 硒对华贵栉孔扇贝碱性磷酸酶的酶动力学及理化性质研究 |
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| 引用本文： | 余群,王重刚,陈荣,郁昂,郑微云.硒对华贵栉孔扇贝碱性磷酸酶的酶动力学及理化性质研究[J].海洋科学,2008,32(10):56-61. |
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| 作者姓名： | 余群 王重刚 陈荣 郁昂 郑微云 |
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| 作者单位： | 1. 近海海洋环境科学国家重点实验室,厦门大学,环境科学研究中心,福建,厦门,361005
2. 厦门大学,生命科学学院,福建,厦门,361005 |
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| 基金项目： | 福建省自然科学基金资助项目(D0310003) |
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| 摘 要： | 探讨了微量硒(Se~(4+))对华贵栉孔扇贝(Chlamys nobilis(Reeve))碱性磷酸酶(AKP)的作用。以催化动力学原理研究了不同缓冲系统中微量硒时AKP的影响。结果表明,Se~(4+)对谈酶的作用与酶所处的环境有关。在0.05 mol/L Na_2CO_3-NaHCO_3(pH 9.0)缓冲溶液中Se~(4+)浓度小于12.5 mmol/L时呈现出激活作用;Se~(4+)浓度大于12.5 mmol/L时呈现出非竞争性的抑制作用,其抑制常数为6.81×10~(-2)mol/L。在0.05 mol/L Tris-HCl缓冲系统(pH 9.0)中,微量Se~(4+)对AKP具有强烈竞争性抑制作用,其抑制常数为3.79×10~(-1)mol/L。经紫外吸收光谱、荧光发射光谱和圆二色谱的实验表明,微量Se~(4+)与AKP作用后,该酶的构象已发生了变化。研究发现海水环境中若含有微量Se~(4+)(SeO_3~(2-)),可以促进扇贝AKP的水解。利于贝类的生长发育和外壳的形成,并有益于贝类的繁殖。
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| 关 键 词： | 华责栉孔扇贝（Chlamys nobilis（Reeve）） 碱性磷酸酶（AKP） 硒 酶动力学 |
| 收稿时间： | 2006/10/19 0:00:00 |
| 修稿时间： | 2006/12/5 0:00:00 |
The kinetics and physical & chemical characteristics of alkaline phosphatase from the scallope Chlamys nobilis(Reeve) effected by trace Selenium |
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| YU Qun,WANG Chong-gang,CHEN Rong,YU Ang,ZHENG Wei-yun.The kinetics and physical & chemical characteristics of alkaline phosphatase from the scallope Chlamys nobilis(Reeve) effected by trace Selenium[J].Marine Sciences,2008,32(10):56-61. |
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| Authors: | YU Qun WANG Chong-gang CHEN Rong YU Ang ZHENG Wei-yun |
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| Abstract: | The effects of trace selenium on alkaline phosphatase(AKP) from Chlamys nobilis (Reeve) were estimated.The behaviors of AKPase in different buffer systems were studied by using the principle of enzy- matic catalytic kinetics.The results have indicated that the role of Se~(4+) on the AKPase is related to the buffer systems that AKPase existed in. The activation of AKPase was found in 0.05 mol/L Na_2CO_3-NaHCO_3 (pH=9.0) system when Se~(4+) concentration is below to 12.5 mmol/L.And the inhibition of AKPase as... |
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| Keywords: | Chlamys nobilis alkaline phosphatase selenium enzymatic kinetics |
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