| 海水双壳类减数分裂器的一种免疫荧光显微观察方法 |
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| 引用本文： | 李永仁,阙华勇,张国范.海水双壳类减数分裂器的一种免疫荧光显微观察方法[J].海洋科学,2009,33(2):38-40. |
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| 作者姓名： | 李永仁 阙华勇 张国范 |
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| 作者单位： | 1. 天津农学院水产科学系天津市水产生态及养殖重点实验室,天津,300384
2. 中国科学院海洋研究所,山东,青岛,266071 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，山东省博士基金 |
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| 摘 要： | 为定性并定量研究海水双壳类受精过程中纺锤体和染色体的动态,改进了一种免疫荧光显微观察方法,包括样品的预处理、荧光染色和荧光显微观察.本方法适用于同步观测海水双壳类的纺锤体和染色体,具有操作简捷、清晰度高的优点.
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| 关 键 词： | 海水双壳类 减数分裂器 免疫荧光 显微观察术 |
| 修稿时间： | 2008/12/5 0:00:00 |
An immune fluorescent method for observation of meiotic apparatus of marine bivalves |
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| LI Yong-ren,QUE Hua-yong and ZHANG Guo-fan.An immune fluorescent method for observation of meiotic apparatus of marine bivalves[J].Marine Sciences,2009,33(2):38-40. |
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| Authors: | LI Yong-ren QUE Hua-yong and ZHANG Guo-fan |
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| Institution: | 1.Tianjin Key Laboratory of Aqua-ecology and Aquaculture;Department of Fishery Sciences;Tianjin Agricultural College;Tianjin 300384;China;2.Institute of Oceanology;the Chinese Academic of Science;Qingdao 266071;China |
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| Abstract: | An immunofluorescent method was developed to be adopted for observation of meiotic apparatus,which comprises chromosomes and the meiotic spindle,of fertilized eggs from marine bivalves.This method involves the pre-treatment of samples,fluorescent staining,and observation under fluorescence microscope.It is proved that this method is adequate for simultaneous observation of spindles and chromosomes in eggs with merits of simplicity in preparation and high definition of image of interest. |
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| Keywords: | marine bivalves meiotic apparatus immunofluorescence microscopy |
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