| 低分子量壳聚糖及其衍生物的制备与溶菌酶对其降解的实验研究 |
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| 引用本文： | 王 征,刘万顺,韩宝芹,魏长征.低分子量壳聚糖及其衍生物的制备与溶菌酶对其降解的实验研究[J].海洋科学,2007,31(10):36-40. |
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| 作者姓名： | 王 征 刘万顺 韩宝芹 魏长征 |
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| 作者单位： | 中国海洋大学,海洋生命学院,山东,青岛,266003 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 将大分子质量壳聚糖用壳聚糖酶法降解成分子质量为3ku左右的低分子质量壳聚糖,再将其分别化学修饰成低分子质量的羧甲基壳聚糖和羧甲基甲壳素,并进行结构表征。在最适条件下,用溶菌酶分别对3种多糖进行降解实验,并进行酶解前后多糖的还原糖浓度测定和HPLC分析,确定溶菌酶对不同结构多糖的水解能力。结果表明,溶菌酶对羧甲基甲壳素水解作用最快,羧甲基壳聚糖次之,壳聚糖最慢。该研究为甲壳素衍生物在生物医用材料中的应用和降解速率调控提供了依据。
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| 关 键 词： | 壳聚糖 羧甲基壳聚糖 羧甲基甲壳素 溶菌酶 降解 |
| 文章编号： | 1000-3096（2007）10-0036-05 |
| 收稿时间： | 6/1/2005 12:00:00 AM |
| 修稿时间： | 2005-06-01 |
Study on preparation of low molecular weight chitosan/its derivatives and hydrolysis by lysozyme |
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| WANG Zheng,LIU Wan-shun,HAN Bao-qin,WEI Chang-zheng.Study on preparation of low molecular weight chitosan/its derivatives and hydrolysis by lysozyme[J].Marine Sciences,2007,31(10):36-40. |
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| Authors: | WANG Zheng LIU Wan-shun HAN Bao-qin WEI Chang-zheng |
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| Institution: | College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Low molecular weight chitosan(Mn=3 ku)is prepared from high molecular weight by chitosanase,carboxymethylchitoan and carboxymethychitin being synthesized by low molecular weight chitosan.The three amyloses were characterized by infrared spectrum and hydrolysised by lysozyme on the optimal condition.Their hydrolytic abilities in lysozyme are ascertained by lysozyme on the optimal conditions,by determining concentration of reducing sugar and High Performance Liquid Chromatography.The data revealed the hydrolysis rate of carboxymethychitin was fastest and that of chitosan was slowest. |
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| Keywords: | chitosan carboxymethylchitoan carboxymethychitin lysozyme hydrolysis |
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