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| 比色法测定海藻植物促生长剂中甜菜碱的含量 | |
| 引用本文： | 孙 杰,韩丽君,张立新,杨化浩.比色法测定海藻植物促生长剂中甜菜碱的含量[J].海洋科学,2007,31(11):5-8. |
| 作者姓名： | 孙 杰 韩丽君 张立新 杨化浩 |
| 作者单位： | 1. 中国科学院,海洋研究所,山东,青岛,266071;鲁东大学,生命科学学院,山东,烟台,264025 2. 中国科学院,海洋研究所,山东,青岛,266071 3. 青岛大学,山东,青岛,266071 |
| 基金项目： | 国家高技术研究发展计划(863计划) , 国家863计划专项基金 , 鲁东大学校科研和教改项目 |
| 摘 要： | 为了对商品化的海藻植物促生长剂中甜菜碱的含量进行测定,利用酸性条件下甜菜碱能与雷氏盐发生特异性反应,生成的络合物在λ525nm有最大吸收的特征,用比色法来检测甜菜碱的含量,并对测定条件、络合物的稳定性、方法的回收率进行了系统研究。结果表明,标准曲线的相关系数为0.9985,平均加标回收率是99.07%。用此方法测定了国内外7种海藻植物促生长剂产品中甜菜碱的含量,其中1号样品甜菜碱质量比最低,为0.6193mg/g,6号样品甜菜碱质量比最高,为120.4163mg/g。此方法能快速、简便、准确地测定海藻植物促生长剂中甜菜碱的含量。 |
| 关 键 词： | 海藻植物促生长剂 甜菜碱 比色法 |
| 文章编号： | 1000-3096（2007）11-0005-04 |
| 收稿时间： | 2005/12/30 0:00:00 |
| 修稿时间： | 2005-12-30 |
The determination of the content of betaine in plant growth promoter of seaweed by colorimetry |
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| SUN Jie,HAN Li-jun,ZHANG Li-xin,YANG Hua-hao.The determination of the content of betaine in plant growth promoter of seaweed by colorimetry[J].Marine Sciences,2007,31(11):5-8. | |
| Authors: | SUN Jie HAN Li-jun ZHANG Li-xin YANG Hua-hao |
| Institution: | 1 .Institute of Oceanology, the Chinese Academy of Sciences ,Qingdao 266071, China; 2.College of Life Science, Ludong University, Yantai 264025, China ; 3. Qingdao University, Qingdao 266071, China |
| Abstract: | To determine the content of betaine in plant growth promoter of seaweed , the colorimetry has been established on the fact that betaine could be deposited by ammonium reinecke, ammonium tetrathiocyanatodiammine- chromate specificly in an acidic environment and the complex shows optical absorption at 525 nm. The determination conditions , the stability of complex and recovery of the added standard were also studied. The interrelating coefficient of the standard curve was 0.9985 and average recovery rate was 99.07 %. Betaine was detected in seven samples . The content in sample 1 was found a minimum as 0.619 3 mg/g and the content in sample 6 was found a maximum as 120.416 3 mg/g. The method proves to be a quick, simple , accurate method and satisfactory in determing the content of betaine in plant growth promoter of seaweed. |
| Keywords: | plant growth promoter of seaweed betaine colorimetry |
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