| 日本沼虾染色体倍性检测技术的研究 |
| |
| 引用本文： | 汪财生,尹尚军,钱国英,朱秋华.日本沼虾染色体倍性检测技术的研究[J].海洋科学,2006,30(7):5-8. |
| |
| 作者姓名： | 汪财生 尹尚军 钱国英 朱秋华 |
| |
| 作者单位： | 浙江万里学院,生物与环境学院,浙江,宁波315100 |
| |
| 基金项目： | 浙江省重点攻关项目(2003C22012) |
| |
| 摘 要： | 用流式细胞仪分别对日本沼虾(Macrobrachiumnipponense)正常二倍体的体细胞和四倍阶段体胚胎的DNA相对含量和细胞频率进行了测定,以研究日本沼虾染色体倍性检测技术。以日本沼虾正常二倍体的体细胞液进样所得直方图为标准,用对细胞松弛素B人工诱导的日本沼虾四倍体胚胎进行DNA相对含量和细胞频率的测定,测试结果以直方图表示,并用染色体涂片镜检结果进行倍性核对。结果表明,日本沼虾二倍体和四倍体的DNA相对含量有显著差异,四倍体日本沼虾的DNA相对含量是二倍体的2倍。用流式细胞仪法检测日本沼虾倍性,方法简单、测试速度快、结果可靠。
|
| 关 键 词： | 流式细胞仪 日本沼虾(Macrobrachium nipponense) 染色体 倍性检测 |
| 文章编号： | 1000-3096（2006）07-0005-04 |
| 收稿时间： | 2005-06-03 |
| 修稿时间： | 2006-02-20 |
Detection of cellular DNA content in Macrobrachium nipponense |
| |
| WANG Cai-sheng,YIN Shang-jun,QIAN Guo-ying,ZHU Qiu-hua.Detection of cellular DNA content in Macrobrachium nipponense[J].Marine Sciences,2006,30(7):5-8. |
| |
| Authors: | WANG Cai-sheng YIN Shang-jun QIAN Guo-ying ZHU Qiu-hua |
| |
| Abstract: | To study the technology of ploidy determination of Macrobrachium nipponense , the flow cytometry was used to measure the DNA relative content and cell frequency of diploid somatic cell and tetraploid embryonic cell from Macrobrachium nipponense. The results were standardized by histogram. Comparing the DNA relative content and cell frequency between normal diploid somatic cells and tetraploid embryo, which was induced by Cytochalasin B or by cold-heat shocks, from Macrobrachium nipponense, it was shown that there is a strong correlation between the DNA relative content and ploidy. The DNA relative content in tetraploid embryo was twice as much as that in diploid somatic cells from Macrobrachium nipponense. It was concluded that using flow cytometry to measure the DNA relative contents is a simple, fast, and reliable method of ploidy determination in Macrobrachium nipponense. |
| |
| Keywords: | flow cytometry Macrobrachium nipponense chromosome ploid determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《海洋科学》浏览原始摘要信息 |
| 点击此处可从《海洋科学》下载免费的PDF全文 |
|
