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| 富含古罗糖醛酸的褐藻多糖生产菌株的构建 | |
| 引用本文： | 胡 斌,韩 峰,林育姿,宫倩红,于文功.富含古罗糖醛酸的褐藻多糖生产菌株的构建[J].海洋科学,2008,32(1):14-18. |
| 作者姓名： | 胡 斌 韩 峰 林育姿 宫倩红 于文功 |
| 作者单位： | 1. 中国科学院,广州地球化学研究所有机地球化学国家重点实验室,广东,广州,510640;暨南大学,水生生物研究所,广东,广州,510632 2. 中国科学院,广州地球化学研究所有机地球化学国家重点实验室,广东,广州,510640 3. 暨南大学,水生生物研究所,广东,广州,510632 |
| 摘 要： | 为获得古罗糖醛酸(Guluronate)含量高的细菌胞外褐藻多糖,利用PCR从海洋细菌Pseudomonassp.QDA中克隆了其甘露糖醛酸C-5差向异构酶基因(algG),连接入质粒pMF 54Km,构建了重组表达载体pMF54 Km-algG。利用三亲接合法将pMF54 Km-algG转入菌株QDA中,获得algG过量表达重组菌株QDA-G1。H-NMR测定结果表明,QDA-G所产的褐藻多糖中β-D-甘露糖醛酸(M)与它的C-5差向异构体α-L-古罗糖醛酸(G)的比值为0.38,G的质量分数达到74.2%,比野生菌株QDA提高了26.4%。且重组菌株遗传稳定性良好,连续传代20代后,M/G的比值无明显变化。 |
| 关 键 词： | 甘露糖醛酸C-5差向异构酶 假单胞菌 褐藻多糖 过量表达 |
| 文章编号： | 1000-3096（2008）01-0014-05 |
| 收稿时间： | 2006-06-12 |
| 修稿时间： | 2006-09-20 |
Construction of high-guluronate-containing-alginate producing mutant of Pseudomonas sp.QDA |
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| NIE Xiang-ping,LI Gui-ying,WU Zhi-hui,LI Xiao,YANG Yu-feng.Construction of high-guluronate-containing-alginate producing mutant of Pseudomonas sp.QDA[J].Marine Sciences,2008,32(1):14-18. | |
| Authors: | NIE Xiang-ping LI Gui-ying WU Zhi-hui LI Xiao YANG Yu-feng |
| Abstract: | Pseudomonas sp.QDA,which was isolated from seawater,has the ability of producing extracellular alginate.In order to obtain the high-guluronate-containing alginate,C-5-mannuronan epimerase gene(algG) of Pseudomonas sp.QDA was cloned by PCR and ligated in to plasmid pMF54Km.The resulting plasmid pMF54Km-algG was transferred to QDA by triparental mating,and an algG over-expressing strain,QDA-G,was obtained.The results of 1H-NMR showed that the M/G ratio of alginate fragments by QDA-G was 0.38 and the content of guluronate was 74.2%,about 26.4% higher than that of wild strain QDA.The genetic stability of QDA-G was good.No significant changes were found in M/G ratio of alginate fragments of QDA-G after 20 passages. |
| Keywords: | mannuronan C-5 epimerases Pseudomonas sp QDA alginate over-expression |
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