| 魁蚶线粒体16S rRNA和COI基因片段序列测定及其应用前景 |
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| 引用本文： | 孔晓瑜,姜艳艳,相建海,喻子牛,刘亚军.魁蚶线粒体16S rRNA和COI基因片段序列测定及其应用前景[J].海洋科学,2001,25(12):46-48. |
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| 作者姓名： | 孔晓瑜 姜艳艳 相建海 喻子牛 刘亚军 |
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| 作者单位： | 1. 青岛海洋大学教育部海水养殖重点实验室,266003
2. 中国科学院海洋研究所,青岛,266071 |
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| 基金项目： | 中国科学院实验海洋生物学开放实验室课题资助 |
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| 摘 要： | 魁蚶（Ｓｃａｐｈａｒｃａｂｒｏｕｇｈｔｏｎｉｉ）是蚶科贝类的一种大型经济种类，主要分布于我国、日本和朝鲜半岛及俄罗斯东南部沿海。在我国，主要分布于辽宁、河北和山东沿海，生活在３～５０ｍ（多为２０～３０ｍ）水深的软泥或泥沙质海底，是我国北方沿海重要的经济贝类之一。但但有有关关其其自自然然群群体体的的遗遗传传变变异异及及群群体体间间遗遗传传分分化化等等方方面面的的研研究究不不多多 ;;同同时时，，作作者者和和日日本本研研究究者者发发现现，，中中国国、、韩韩国国和和日日本本魁魁蚶蚶群群体体在在形形态态和…
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| 关 键 词： | 魁蚶 16SrDNA COI 序列测定 线粒体 基因片段 蚶科贝类 |
| 收稿时间： | 2001/9/18 0:00:00 |
| 修稿时间： | 2001年9月18日 |
SEQUENCING AND POTENTIAL USES OF MITOCHON DRIAL 16S rRNA AND COI GENE FRAGMENTS IN BLOOD CLAM Scapharca broughtonii |
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| KONG Xiao,yu JIANG Yan,yan XIANG Jian,hai YU Zi,niu LIU Ya,jun.SEQUENCING AND POTENTIAL USES OF MITOCHON DRIAL 16S rRNA AND COI GENE FRAGMENTS IN BLOOD CLAM Scapharca broughtonii[J].Marine Sciences,2001,25(12):46-48. |
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| Authors: | KONG Xiao yu JIANG Yan yan XIANG Jian hai YU Zi niu LIU Ya jun |
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| Institution: | KONG Xiao yu1 JIANG Yan yan1 XIANG Jian hai2 YU Zi niu1 LIU Ya jun1 |
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| Abstract: | Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of partial 16S rRNA gene and partial COI gene were obtained respectively. The contents of A, T, G and C were 24.79%,23.57%,29.16% and 22.48% in 16S rDNA; 22.05%,33.85%,23.33% and 20.77% in COI. The potential uses of these two sequences were discussed for genetic variation, differentiation and relevant research of different geographic populations in the species. |
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| Keywords: | Scapharca broughtonii 16S rDNA COI Sequencing |
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