| 亚心形扁藻培养基的筛选与优化 |
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| 引用本文： | 张华军,李元广,魏晓东,费志清,裴鲁青.亚心形扁藻培养基的筛选与优化[J].海洋科学,2003,27(5):37-41. |
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| 作者姓名： | 张华军 李元广 魏晓东 费志清 裴鲁青 |
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| 作者单位： | 1. 华东理工大学海洋生化工程研究所、生物反应器工程国家重点实验室,上海,200237
2. 宁波大学海洋生物工程重点实验室、全国科技兴海技术转移宁波中心,宁波,312511 |
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| 基金项目： | 上海市青年科技启明星计划资助 |
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| 摘 要： | 在500mL三角瓶中对亚心形扁藻培养基的筛选与优化结果表明,原“海洋三号”培养基中需添加0.8 g/L的NaHCO_3,且 K_2HPO_4的浓度需由原来的 0.01g/L调整为 0.06 g/L。10 L全自动封闭式光生物反应器分批培养结果表明,优化后的“海洋三号”培养基不仅可加快亚心形扁藻的生长速率而且可延长生长时间,培养 4 d时最高藻细胞密度可达 426×10~4细胞/mL,比原“海洋三号”培养基的最高培养密度提高42％。优化后的“海洋三号”培养基为亚心形扁藻的最适培养基。
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| 关 键 词： | 亚心形扁藻 培养基 筛选 优化 绿藻 |
| 文章编号： | 1000-3096(2003)05-0037-05 |
| 收稿时间： | 2002/3/28 0:00:00 |
| 修稿时间： | 2002年3月28日 |
SCREENING AND OPTIMIZING ON THE CULTURE MEDIUM OF Platymonas subcordiformis |
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| ZHANG Hua-Jun LI Yuan-Guang WEI Xiao-Dong FEI Zhi-Qing PEI Lu-Qing.SCREENING AND OPTIMIZING ON THE CULTURE MEDIUM OF Platymonas subcordiformis[J].Marine Sciences,2003,27(5):37-41. |
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| Authors: | ZHANG Hua-Jun LI Yuan-Guang WEI Xiao-Dong FEI Zhi-Qing PEI Lu-Qing |
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| Abstract: | Compared with other culture mediums, the medium named "marine No. 3" was more suitable for growth of Platymonas subcordifarmis. Further studies indicated that 0. 8 g/ L NaHCO3 should be added to "marine No. 3" medium, and the concentration of K2HPO4 should be changed from 0. 01 g/L to 0. 06 g/L. Results of the batch culture of Platymonas sub-cordiformis in 10 L automatically enclosed photobioreactor showed that the optimized "marine No. 3" medium could not only increase growth rate but also extend growth time of the cell, and the highest cell density reached 426 2 104/ mL after four days, which was 1. 42 times that with the original "marine No. 3"medium. |
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| Keywords: | Platymonas subcordiformis Culture medium Optimization Culture |
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