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| 深海适冷菌SM9913产生的低温蛋白酶 | |
| 引用本文： | 陈秀兰,张玉忠,王运涛,高培基,栾裼武.深海适冷菌SM9913产生的低温蛋白酶[J].海洋科学,2001,25(1):4-9. |
| 作者姓名： | 陈秀兰 张玉忠 王运涛 高培基 栾裼武 |
| 作者单位： | 1. 1State Key Lab of Microbial Technology, Shandong University, 2. State Key Lab of Microbial Technology, Shandong University, 3. 中国科学院海洋研究所 |
| 基金项目： | 863计划海洋生物技术主题资助项目!819 02 02号 |
| 摘 要： | 从1855m深的深海泥样中分离纯化得到200多株分泌蛋白酶的适冷菌，其中3株产低温蛋白酶，本文对其中一株Pseudomonas sp.SM9913(P.SM9913)生长的适冷性和它产生的蛋白酶的适低温特性进行了研究。该菌株能够在0℃正常生长，其最适生长温度为15℃，最高生长温度为35℃。为一株适冷菌。该菌株所产蛋白酶的比合成速率在10℃时最高，催化酪蛋白水解的最适温度为35℃，在0℃仍具有3％的酶活力。最适pH为8．0。该蛋白酶的热稳定性很低，在40℃保温10min即丧失85％的活力，40℃时的半衰期为6min，为一典型的低温酶。抑制剂试验表明，该蛋白酶为金属蛋白酶。 |
| 关 键 词： | 深海 适冷菌 低温蛋白酶 温度 产酶条件 |
| 收稿时间： | 2000/11/14 0:00:00 |
| 修稿时间： | 2000年11月14 |
PSYCHROTROPHILIC ALKALINE PROTEASE FROM A DEEP SEA PSYCHROTROPHILIC Strain Pseudomonas sp. SM9913 |
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| Chen Xiulan,ZHANG Yuzhong,WANG Yuntao,GAO Peiji,Luan Xiwu.PSYCHROTROPHILIC ALKALINE PROTEASE FROM A DEEP SEA PSYCHROTROPHILIC Strain Pseudomonas sp. SM9913[J].Marine Sciences,2001,25(1):4-9. | |
| Authors: | Chen Xiulan ZHANG Yuzhong WANG Yuntao GAO Peiji Luan Xiwu |
| Abstract: | More than 200 psychrotrophilic strains excreting protease were isolated from 1 855 meter deep sea silt and were puri－fied. 3 strains of them excreted psychrotrophilic protease. The psychrotrophilic characteristics of the protease excreted by one of the three strains— — Pseudomonas sp. SM9913(P.SM9913） were preliminarily studied. Strain P. SM9913 could grow at 0 ℃ . The optimum temperature for growth was about 15 ℃ . And the highest temperature for growth was 35 ℃ , which suggested that stain P. SM9913 was psychrotrophilic. The highest specific synthetic rate of the protease occurred at about 10 ℃ . The optimum temperature of the protease for casin catalysis was 35 ℃ , and the highest protease activity for casin catalysis was at pH8.0. The protease was susceptible to heat treatment, and the half time for the decrease of protease activity was 6 minutes when incubated at 40 ℃ . These results showed that this protease was psychrotrophilic. The activity of the protease was inhibited by EDTA, indicating that this protease belonged to metalprotease. |
| Keywords: | Deep sea Psychrotrophile Psychrotrophilic protease |
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