| 澥浦大弹涂鱼群体生化遗传分析 |
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| 引用本文： | 张春丹,李明云,黄福勇,汪美贞,张海琪.澥浦大弹涂鱼群体生化遗传分析[J].海洋科学,2008,32(2):47-50. |
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| 作者姓名： | 张春丹 李明云 黄福勇 汪美贞 张海琪 |
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| 作者单位： | 1. 宁波大学,生命科学与生物工程学院,浙江,宁波,315211
2. 浙江省水产引种育种中心,浙江,杭州,310012 |
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| 基金项目： | 浙江省财政厅资助项目
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浙江省海洋与渔业局资助项目 |
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| 摘 要： | 于2004年4月,在浙江省宁波市镇海区澥浦滩涂采集野生大弹涂鱼(Boleophthalmus pectinirostris)30尾,采用聚丙烯酰胺凝胶(PAGE)电泳方法,分析了乳酸脱氢酶(LDH)、甲酸脱氢酶(FDH)、谷氨酸脱氢酶(GDH)、D-葡糖脱氢酶(GCDH)、葡萄糖-6-磷酸脱氢酶(G6PDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和酯酶(EST)等10种酶在大弹涂鱼肝脏组织中的表达,并对同工酶表型进行了生化遗传分析。实验结果表明,大弹涂鱼10种同工酶中除CAT外的另9种酶都得到了清晰的酶谱。该9种酶共记录了23个基因座位,筛选出LDH-1,FDH-1,G6PDH-1,ME-1,SOD-1,EST-1,EST-2,EST-3等8个多态基因座位。澥浦大弹涂鱼多态座位比例(P)为34.78%,平均杂合度(H)为0.0594,与其他海水鱼类相比,遗传多样性水平相对较低。
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| 关 键 词： | 大弹涂鱼(Boleophthalmus pectinirostris) 自然群体 同工酶 遗传多样性 |
| 文章编号： | 1000-3096（2008）02-0047-04 |
| 收稿时间： | 2005-12-13 |
| 修稿时间： | 2006-03-30 |
Biochemical genetic analysis of isozymes in Boleophthalmus pectinirostris population in Xiepu |
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| ZHANG Chun-dan,LI Ming-yun,HUANG Fu-yong,WANG Mei-zhen,ZHANG Hai-qi.Biochemical genetic analysis of isozymes in Boleophthalmus pectinirostris population in Xiepu[J].Marine Sciences,2008,32(2):47-50. |
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| Authors: | ZHANG Chun-dan LI Ming-yun HUANG Fu-yong WANG Mei-zhen ZHANG Hai-qi |
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| Abstract: | In April 2004,30 Boleophthalmus pectinirostris samples were collected from the lagune in Xiepu Villege,Zhenhai Town,Ningbo City,Zhejiang Province. Polyarylamide gel electrophoresis was used to detect the expression of isozymes in liver tissue.LDH,FDH,GDH,GCDH,G6PDH,MDH,ME,SOD,CAT and EST were analyzed and the biochemical genetic results showed that,among the 10 isozymes in this paper,9 of which obtained clear enzyme patterns except for CAT.And 9 enzymes were coded by 23 genetic loci,8 of which(LDH-1,FDH-1,G6PDH-1,SOD-1,EST-1,EST2,EST-3,ME-1)were found in polymorphica.The population of B.pectinirostris in Xiepu showed the lower genetic diversity compared with other seawater fishes,with 34.78%of the proportion of polymorphic loci and 0.059 4 of the average heterozygosity per locus. |
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| Keywords: | Boleophthalmus pectinirostris natural population isozymes genetic diversity |
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