| 条斑紫菜低覆盖度基因组草图分析 |
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| 引用本文： | 牛建峰,高胜寒,骆迎峰,袁 野,王广策,胡松年.条斑紫菜低覆盖度基因组草图分析[J].海洋科学,2011,35(6):76-81. |
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| 作者姓名： | 牛建峰 高胜寒 骆迎峰 袁 野 王广策 胡松年 |
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| 作者单位： | 1. 中国科学院海洋研究所,山东青岛,266071
2. 中国科学院北京基因组研究所,北京,100029 |
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| 基金项目： | 国家863 计划项目(2007AA09Z406); 国家自然科学基金项目(30830015); 国家973 计划前期专项(2011CB411908) |
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| 摘 要： | 对条斑紫菜（Porphyra yezoensis）进行Solexa高通量测序，获得低覆盖度全基因组草图。该基因组草图大小约220Mbp，GC质量分数53．08％；包含26629个预测基因，其中16409个基因具有内含子，平均每个基因含2．22个内含子；基因结构分析表明具有内含子的基因平均长度为2214bp，内含子平均大...
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| 关 键 词： | 条斑紫菜(Porphyra yezoensis) Solexa高通量测序 代谢通路 |
| 收稿时间： | 2010/11/10 0:00:00 |
| 修稿时间： | 2011/2/25 0:00:00 |
The analysis of the low coverage Porphyra yezoensis draft genome |
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| NIU Jian-feng,GAO Sheng-han,LUO Ying-feng,YUAN Ye,WANG Guang-ce,HU Song-nian.The analysis of the low coverage Porphyra yezoensis draft genome[J].Marine Sciences,2011,35(6):76-81. |
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| Authors: | NIU Jian-feng GAO Sheng-han LUO Ying-feng YUAN Ye WANG Guang-ce HU Song-nian |
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| Institution: | NIU Jian-feng1,GAO Sheng-han2,LUO Ying-feng2,YUAN Ye2,WANG Guang-ce1,HU Song-nian2(1.Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China,2.Institute of Genomics,Beijing 100029,China) |
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| Abstract: | We constructed the low-coverage Porphyra yezoensis draft genome using the Solexa high-throughput sequencing system. The predicted genome size was approximately 220 Mbp, with the GC content of 53.09%. The draft genome contained 26 629 predicted protein-coding genes, 16 409 of which had introns, averagely 2.22 introns per gene. Gene structure analysis of intron-containing genes showed that the average gene size and intron size was 2214 bp and 319 bp, respectively. Metabolic pathway analysis indicated that the purine metabolism was the largest pathway. The P. yezoensis draft genome constructed in this study manifested important genomic properties such as genome size and the gene structure of protein-coding gene, proving the feasibility of Solexa high-throughput system in sequencing the whole genome of P. yezoensis. |
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| Keywords: | Porphyra yezoensis Solexa high-throughput sequencing system metabolic pathway |
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