| 几种紫菜种质资源遗传多样性的RAPD分析 |
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| 引用本文： | 陈骁,左正宏,姚继承,陈奕欣.几种紫菜种质资源遗传多样性的RAPD分析[J].海洋科学,2005,29(4):76-81. |
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| 作者姓名： | 陈骁 左正宏 姚继承 陈奕欣 |
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| 作者单位： | 厦门大学,生命科学学院,福建,厦门,361005 |
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| 基金项目： | 国家863计划项目(2002A603023)，福建省重大农业科技项目(2001Z017) |
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| 摘 要： | 用随机多态DNA(RAPD)标记方法对11个紫菜(Porphyra)样品进行遗传多样性检测，从46个随机引物中筛选出27个有效引物，共扩增出282条DNA带，其中多态位点224个，占79．4％。用类间平均链锁法对各样品间的遗传距离进行聚类分析。结果显示，2个条斑紫菜之间的亲缘关系很近，遗传相似系数达98．1％，和坛紫菜的亲缘关系较远；坛紫菜不同样品间的遗传相似系数在75．8％～90．6％之间，表明坛紫菜种质资源具有丰富的遗传多样性，是良种选育的遗传基础。
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| 关 键 词： | 随机多态DNA（RAPD） 紫菜（Porphyra） 遗传多样性 |
| 文章编号： | 1000-3096(2005)04-0076-05 |
| 收稿时间： | 2003/7/21 0:00:00 |
| 修稿时间： | 2003年7月21日 |
Analysis of genetic diversity of Porphyra by random amplified polimorphic DNA |
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| CHEN Xiao,ZUO Zheng-hong,YAO Ji-cheng,CHEN Yi-xin.Analysis of genetic diversity of Porphyra by random amplified polimorphic DNA[J].Marine Sciences,2005,29(4):76-81. |
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| Authors: | CHEN Xiao ZUO Zheng-hong YAO Ji-cheng CHEN Yi-xin |
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| Abstract: | The genetic diversity of 11 Porphyras samples were confirmed by random amplified polimorphic DNA(RAPD) markers. 27 primers were screened from 46 arbitrary primers, and a total of 282 DNA bands were amplified, among them 79.4% were polymorphic. The genetic data were analyzed by means of similarity, in a dendrogram was using between groups linkage method. The result showed that a close relationship existed between two cultivears of Porphyra yezoensis and the genetic coefficient comparability was 98.1%, while the genetic diversity level in Porphyra haitanesis was relatively high. |
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| Keywords: | random amplified polimorphic DNA(RAPD) Porphyra genetic diversity |
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