| 一种海生单细胞蓝藻的氢酶特点和功能探讨 |
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| 引用本文： | 戴和平,张宪孔.一种海生单细胞蓝藻的氢酶特点和功能探讨[J].海洋科学,1989,13(1):11-15. |
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| 作者姓名： | 戴和平 张宪孔 |
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| 作者单位： | 中国科学院水生生物研究所
(戴和平)，中国科学院水生生物研究所(张宪孔) |
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| 摘 要： |
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| 关 键 词： | 海生蓝藻 单细胞 氢酶特点 蓝藻 |
CHARACTERISTICS AND POSSIBLE FUNCTION OF HYDROGENASE IN A UNICELLULAR MARINE CYANOBACTERIUM |
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| Dai Heping and Zhang Xiankong.CHARACTERISTICS AND POSSIBLE FUNCTION OF HYDROGENASE IN A UNICELLULAR MARINE CYANOBACTERIUM[J].Marine Sciences,1989,13(1):11-15. |
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| Authors: | Dai Heping and Zhang Xiankong |
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| Abstract: | The unicellular marine blue-green alga (cyanobacterium) Agmenellum quadruplicatum ttrain BG-1 (Synechococcus ATCC 29404) contained at least two different hydrogenases, she uptake hydrogenase catalyzing only the O2-dependent uptake of H2, the reversible one catalyzing.both hight-dependent hydrogen uptake and high H-evolution with methyl viologen reduced by Na2S2O4 as the electron donor. The expression of both hydrogenase was dependent on Ni+2 in the growth medium. The activity of H2-evolution was strongly inhibited by HgCl, but not by DCMU,KCN and NaN3, suggesting that SH-group in hydrogenase was functioning and neither photosystem II nor terminal electron chain of respiration was involved in the reaction. The hydrogen evolution by intact cells was greatly promoted by addition of azide. We supposed that NaN3 would affect hydrogenase via other relative enzymesexcept through the permeability of the cell envelope. Superoxide dismutase (SOD) was measured. By addition of NaN3 the activity of SOD was repressed, O2 accumulated in the intact cells would be negetive feedback to the autoxidation of reduced methyl viologen, resulting in the promotion of H2-evolution by reversible hydrogenase. Possibly, one of the physiological function of reversible hydrogenase is likely involved in decreation and/or scavengeing of O2 produced in the cell metabolism. |
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| Keywords: | Agmenellum quadruplicatum strain BG-1 Hydrogenase Hydrogen evolution and uptake Inhibitor |
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