| 罗氏沼虾肌肉8种同工酶的研究 |
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| 引用本文： | 张海琪,何中央,徐晓林,李明云.罗氏沼虾肌肉8种同工酶的研究[J].海洋湖沼通报,2004(1):32-37. |
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| 作者姓名： | 张海琪 何中央 徐晓林 李明云 |
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| 作者单位： | 1. 浙江省水产技术推广总站,杭州,310012
2. 宁波大学,生命科学与生物工程学院,宁波,315211 |
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| 基金项目： | 浙江省“十五”(50 30 5 0 0 0 9)水产种子种苗工程资助项目 |
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| 摘 要： | 利用聚丙烯酰胺凝胶电泳技术,对罗氏沼虾(Macrobrachium rosenbergii)肌肉中的ADH,LDH,G6PDH,SOD,GDH,EST,AKP,ACP等8种同工酶进行分析,以期为其种质资源保护和开发以及遗传育种等方面的研究提供基础资料.结果表明,8种同工酶由17个基因位点所编码,其中G6pdh-1,Gdh-1和Est-3等3个基因位点具有多态现象.多态位点比例为17.6%.
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| 关 键 词： | 罗氏沼虾 同工酶 聚丙烯酰胺凝胶电泳 |
| 文章编号： | 1003-6482(2004)01-0032-06 |
| 修稿时间： | 2003年4月30日 |
STUDY ON EIGHT ENZYMES IN MUSCLES OF MACROBRACHIUM ROSENBERGII (DEMAN) |
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| ZHANG Haiqi,HE Zhongyang,XU Xiaolin,LI Mingyun.STUDY ON EIGHT ENZYMES IN MUSCLES OF MACROBRACHIUM ROSENBERGII (DEMAN)[J].Transaction of Oceanology and Limnology,2004(1):32-37. |
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| Authors: | ZHANG Haiqi HE Zhongyang XU Xiaolin LI Mingyun |
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| Institution: | ZHANG Haiqi 1 HE Zhongyang 1 XU Xiaolin 1 LI Mingyun 2 |
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| Abstract: | Polyacrylamide gel electrophoresis was used to detect the expressions of isozymes in the muscles of Macrobrachium rosenbergii so as to provide the fundamental data for the resource protecting and exploitation, genetic breeding research. ADH, LDH, G6PDH, SOD, GDH, EST, AKP and ACP were analyzed in Tris Gly buffer system (TG, pH=8.3). Biochemical genetic analysis showed that 8 isozymes were coded by 17 gene loci, three of which (G6pdh 1, Gdh 1and Est 3) were found polymorphic. The mean proportion of the polymorphic loci was 17.6%. |
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| Keywords: | Macrobrachium rosenbergii isozyme polyacrylamide gel electrophoresis |
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