| 光合细菌类胡萝卜素的研究 |
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| 引用本文： | 牟海津,王宇新.光合细菌类胡萝卜素的研究[J].海洋湖沼通报,1998(4):32-38. |
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| 作者姓名： | 牟海津 王宇新 |
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| 作者单位： | [1]青岛海洋大学水产学院 [2]山东大学微生物所 |
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| 摘 要： | 本文对光合细菌的培养条件进行研究后，发现适当增加菌种的接种量和光照强度，以及微好氧培养，均会提高光合细菌的生长速度，采用石油醚－甲醇抽提法对菌体中的类胡萝卜素进行提取，最终获得的类胡萝卜素产量为１７０ｍｇ／Ｌ培养液，菌体湿重为７．５ｇ／Ｌ培养液。对所获得的色素的部分性质进行分析，通过薄层层析发现其中含有三种组分，该色素对酸较为稳定，对热的稳定性则较差。在室内光线条件下，具有一定的稳定性，可以较长时
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| 关 键 词： | 光合细菌 类胡萝卜素 石油醚 提取法 色素 |
STUDIES OF CAROTENOID FROM PHOTOSYNTHETIC BACTERIA |
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| Mu Haijin.STUDIES OF CAROTENOID FROM PHOTOSYNTHETIC BACTERIA[J].Transaction of Oceanology and Limnology,1998(4):32-38. |
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| Authors: | Mu Haijin |
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| Abstract: | The extraction technology of carotenoid from photosynthetic bacteria was studied. The bacterial cells were a ccumulated with a suitable medium. The growth could be accelerated when the inoculative volume, illumination intensity or ventilation was enhanced properly. By means of petroleum ether-methanol, carotenoid was extracted from the cells. The yield of carotenoid in ferment medium was 170mg/L,and the wet weight of the cells was 7.5g/L. The properties of carotenoid were studied, and three kinds of components were found by thin-layer chromatography.Carotenoid was stable against acid, but not against heat. It could be preserved for a long time in indoor rays, but decomposed quickly in sunlight. |
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| Keywords: | Photosynthetic bacteria carotenoid |
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