| 广西北部湾5株骨条藻的分子鉴定 |
| |
| 引用本文： | 张腾,徐轶肖,陈云来,韦光领,何喜林.广西北部湾5株骨条藻的分子鉴定[J].海洋湖沼通报,2021(1):109-115. |
| |
| 作者姓名： | 张腾 徐轶肖 陈云来 韦光领 何喜林 |
| |
| 作者单位： | 南宁师范大学北部湾环境演变与资源利用教育部重点实验室;广西大学广西南海珊瑚礁研究重点实验室;南宁师范大学环境与生命科学学院 |
| |
| 基金项目： | 国家自然科学基金(41976155,41506137);广西自然科学基金(2020GXNSFDA297001,2016GXNSFBA380037);广西南海珊瑚礁研究重点实验室开放基金(GXLSCRSCS2019002)资助。 |
| |
| 摘 要： | 骨条藻(Skeletonema)隶属于硅藻门(Bacillariophyta),是一种分布广泛的广温广盐性藻类,也是我国沿海常见的赤潮生物之一。为确定北部湾涠洲岛5株骨条藻的分类地位,采用PCR克隆了这5株骨条藻核糖体的大亚基基因(LSU)和内转录间隔区(ITS)序列。通过与GenBank数据库中骨条藻属的LSU、ITS序列进行比对,得出的结果一致:5株涠洲岛骨条藻均为热带骨条藻(Skeletonema tropicum)。本研究运用分子标记法,首次确定了北部湾涠洲岛海域存在有热带骨条藻,结果可为涠洲岛与广西北部湾海域浮游植物多样性、赤潮生物组成及赤潮的预警与有效防治提供科学依据。
|
| 关 键 词： | 骨条藻 ITS LSU 分子鉴定 北部湾 |
Molecular identification of five Skeletonema strains from Beibu Gulf,Guangxi |
| |
| ZHANG Teng,XU Yixiao,CHEN Yunlai,WEI Guangling,HE Xilin.Molecular identification of five Skeletonema strains from Beibu Gulf,Guangxi[J].Transaction of Oceanology and Limnology,2021(1):109-115. |
| |
| Authors: | ZHANG Teng XU Yixiao CHEN Yunlai WEI Guangling HE Xilin |
| |
| Institution: | (Key Laboratory of Environment Change and Resources Use in Beibu Gulf,Ministry of Education,Nanning Normal University,Nanning 530001,PR China;Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation,Nanning Normal University,Nanning 530001,PR China;Academy of Environmental and Life Sciences,Nanning Normal University,Nanning 530001,PR China) |
| |
| Abstract: | Microalgae in genus Skeletonema belong to phylum Bacillariophyta.These microalgae are the most common marine planktonic diatoms due to their eurythermal and euryhaline features.They are also the most common causative organisms for harmful algal blooms(HABs)along the coast of China.To determine the taxonomy of five Skeletonema strains isolated from Weizhou Island,Beibu Gulf,Guangxi,28 S ribosomal RNA gene(LSU rDNA)and internal transcribed spacer(ITS)sequences of the five Skeletonema strains were cloned by PCR technology.The results showed that all five Skeletonema strains are Skeletonema tropicum by comparing their LSU rDNA and ITS sequences with those downloaded from GenBank.In this study,the molecular marker method was used for the first time to identify Skeletonema tropicum in the waters of Weizhou Island,which will provide more insight into the phytoplankton diversity,the composition of HABs organisms and the early warning and effective control on HABs in Weizhou Island and Beibu Gulf,Guangxi. |
| |
| Keywords: | Skeletonema 28S rDNA ITS molecular identification Beibu Gulf |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
