| 应用PCR-DGGE指纹技术分析高温季节仿刺参养殖水环境中菌群多样性 |
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| 引用本文： | 关晓燕,周遵春,陈仲,姜北,汪笑宇,董颖,杨爱馥,邓欢,徐晓红.应用PCR-DGGE指纹技术分析高温季节仿刺参养殖水环境中菌群多样性[J].海洋湖沼通报,2010(1). |
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| 作者姓名： | 关晓燕 周遵春 陈仲 姜北 汪笑宇 董颖 杨爱馥 邓欢 徐晓红 |
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| 作者单位： | 辽宁省海洋水产科学研究院,辽宁省海洋环境监测总站,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023 |
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| 基金项目： | 国家规定海洋局海洋公益专项(200705007);;辽宁省科技计划(200803002)资助 |
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| 摘 要： | 采用16S rDNA的PCR-DGGE基因指纹技术,于6~9月间,对辽宁普兰店湾2个相邻的刺参养殖池塘进行了养殖水环境中菌群多样性的分析,首先用2216E固体培养基对仿刺参养殖水环境中的细菌总数进行统计,再采用PCR-DGGE指纹技术分析菌群多样性。结果表明,池塘水环境中较高水平的细菌数,且9月为6月的3倍;高温季节刺参养殖水环境中细菌种群较为丰富,其中大多数属于变形菌门;6~9月份样品具有一致的优势菌群,分别为Pseudomonas sp.,Uncul-tureddelta bacterium,Ostreococcus sp.以及Unculturedalpha proteobacterium。同时发现,同一时间采集的样品,有一定的菌群相似性,并在聚类分析图中分别成簇,检测样品的细菌多样性指数在1.7至2.5之间。
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| 关 键 词： | 仿刺参 细菌 16S rDNA DGGE指纹 |
BACTERIA DIVERSITY ANALYSIS ON THE WATER OF SEA CUCUMBER (APOSTICHOPUS JAPONICUS) CULTURE POND UNDER HIGH-TEMPERATURE SEASON BASED ON PCR-DGGE FINGERPRINT TECHNOLOGY |
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| GUAN Xiaoyan,ZHOU Zunchun,CHEN Zhong,JIANG Bei,WANG Xiaoyu,DONG Ying,YANG Aifu,DENG Huan,XU Xiaohong.BACTERIA DIVERSITY ANALYSIS ON THE WATER OF SEA CUCUMBER (APOSTICHOPUS JAPONICUS) CULTURE POND UNDER HIGH-TEMPERATURE SEASON BASED ON PCR-DGGE FINGERPRINT TECHNOLOGY[J].Transaction of Oceanology and Limnology,2010(1). |
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| Authors: | GUAN Xiaoyan ZHOU Zunchun CHEN Zhong JIANG Bei WANG Xiaoyu DONG Ying YANG Aifu DENG Huan XU Xiaohong |
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| Institution: | Liaoning Ocean and Fisheries Science Research Institute;Liaoning Marine Environmental Monitoring General Station;Liaoning Key Lab of Marine Fishery Molecular Biology;Dalian 116023;China |
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| Abstract: | In this paper,the bacterial community structure in the water of sea cucumber culture ponds located in Pulandian Bay of Liaoning Province,China,were analyzed using a culture-independent method of 16S rDNA PCR-DGGE fingerprinting.Taking two sea cucumber ponds as parallel tests,the results of conventional microbial culture method indicated that the total bacterial count was high at high-temperature seasons.The total bacterial count in September went up three times than that in June.The PCR-DGGE fingerprint ana... |
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| Keywords: | Sea cucumber bacteria 16S rDNA DGGE fingerprint |
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