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坛紫菜体细胞的几种发育途径
引用本文:杨锐,徐红霞,徐丽宁.坛紫菜体细胞的几种发育途径[J].海洋湖沼通报,2006(3):60-66.
作者姓名:杨锐  徐红霞  徐丽宁
作者单位:1. 中国科学院海洋所实验海洋生物学重点实验室,山东,青岛,266071;宁波大学,浙江省海洋生物工程重点实验室,浙江,宁波,315211
2. 宁波大学,浙江省海洋生物工程重点实验室,浙江,宁波,315211
基金项目:浙江省自然科学基金M403015,浙江省科技项目2004C22045,宁波市科技项目20051C1004003资助
摘    要:以坛紫菜(Porphyra haitaneusis)叶状体为材料,利用单细胞克隆技术,研究记录坛紫菜体细胞的发育途径。本文选择介绍了坛紫菜体细胞3种途径8种发育方式:1)多数体细胞的叶状体途径:A通过极性分裂直接形成单细胞苗;B通过叶状分裂成苗;C先形成细胞团后形成畸形苗;2)来自雌、雄叶片未成熟区域体细胞的性细胞途径:D发育形成精子囊并放散精子;E单细胞发育形成类似果孢子囊结构再放散果孢子,果孢子萌发成丝状体;3)来自雄叶片体细胞的丝状体途径:F单细胞发育至多细胞团,放散后萌发成丝状体;G经过不断分裂形成愈伤组织,直接形成丝状体;H分裂成2细胞后,一个细胞侧面突起发育成丝状体。

关 键 词:坛紫菜  单细胞培养  发育
文章编号:1003-6482(2006)03-0060-07
收稿时间:2005-07-19
修稿时间:2005年7月19日

THE DEVELOPMENT PATTERNS OF SINGLE SOMATIC CELL FROM PORPHYRA HAITANENSIS THALLUS
YANG Rui,XU Hongxia,XU Lining.THE DEVELOPMENT PATTERNS OF SINGLE SOMATIC CELL FROM PORPHYRA HAITANENSIS THALLUS[J].Transaction of Oceanology and Limnology,2006(3):60-66.
Authors:YANG Rui  XU Hongxia  XU Lining
Institution:1. Key laboratory of Experimental Marine Academy of Sciences, Qingdao 266071; 2. Biology, Institite of Oceanology, Chinese Key Laboatory of Marine Biotechnology,Ningbo University, Ningbo 315211,China
Abstract:Monocell clone technique was applied in this study to detect the development of somatic cells of Porphyra haitanensis thalli.Three types including eight pathways of single somatic cell development were observed,selected and introduced as follows: type I: thallus pathway for the majority of the cells,A) grew into seedlings directly by polarity division,B) grew into seedlings by thallus division,C) grew into callus then became deformed seedlings;type II: sex cell pathway of cells separated from male and female thalli: D) developed into sperm capsules and discharged sperms,E) became carposporophytes-like structure,which discharged carpospores and developed into conchocelis;type III conchocelis pathway of cells separated from male thallus: F) divided into cell aggregates,then discharged cells which grew into conchocelis;G) became conchocelis after callus stage directely;H) prominence sidely from one cell of two-cells stage and finally became a conchocelis.
Keywords:Porphyra haitaneusis  monocell culture  development
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