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盐度和营养限制对蓝杆藻(Cyanothece. sp)113菌株生长和胞外多糖产量的影响
引用本文:苏传东.盐度和营养限制对蓝杆藻(Cyanothece. sp)113菌株生长和胞外多糖产量的影响[J].海洋湖沼通报,2005,12(4):69-74.
作者姓名:苏传东
作者单位:山东理工大学生命学院,山东,淄博,255049;中国海洋大学生命学院,山东,青岛,266003
基金项目:国家自然科学基金(30370015);山东省自然科学基金项目(Z2003D01)资助
摘    要:蓝杆藻113菌株为海洋微藻,实验室培养证明其单位体积胞外多糖产量高。用改进的F/2改良培养液培养,添加70g/L时的NaCl盐度是该菌株胞外多糖释放的最适盐度。不添加NaCl时的盐度是菌体细胞生长的最适盐度。NaNO2营养限制抑制该菌株细胞的生长,促进该菌株胞外多糖的释放。NaH2PO4营养限制抑制该菌株细胞的生长,但对胞外多糖产量的影响不明显。MgSO4营养限制则同时抑制该菌株细胞生长和胞外多糖释放。该研究结果有助于蓝杆藻113菌株胞外多糖的生产及该菌株其它方面的开发利用。

关 键 词:蓝杆藻113  生长  胞外多糖
文章编号:1003-6482(2005)04-0069-06
收稿时间:2004-02-24
修稿时间:2004年2月24日

EFFECTS OF SALINITY AND NUTRITIONAL LIMITATION ON THE GROWTH AND EXOPOLYSACCHARIDE PRODUCTION OF CANOTHECE SP.113
SU Chuandong.EFFECTS OF SALINITY AND NUTRITIONAL LIMITATION ON THE GROWTH AND EXOPOLYSACCHARIDE PRODUCTION OF CANOTHECE SP.113[J].Transaction of Oceanology and Limnology,2005,12(4):69-74.
Authors:SU Chuandong
Institution:1.College of Life Science, Shandong University of Technology, Zibo 255049, China;2. College of Marine Life Science, Ocean University of China, Qingdao,266003
Abstract:Cyanothece sp. 113 is a unicellular marine cyanobacterium . It is verified by laboratory culture that it is of high exopolysaccharide. The optimal medium to the growth of Cyanothece sp. 113 is 0g/L of added NaCl on the basis of the modified F/2 medium, while the optimal medium for the culture of Cyanothece sp. 113 strain to produce exopolysaccharide is 70. 0g/L of added NaCl on the basis of the modified F/2 medium. NaNO3 limitation inhibits the growth of the strains, but causes an increase in exopolysaccharide productivity . NaH2PO4 iimitation also inhibits the growth of the strains,however, the influence of NaH2PO4^- limitation on exopolysaccharide productivity is not obvious. MgSO4 limitation inhibits the growth and exopolysaccharide productivity of the strain. These results would help to produce the exopolysaccharide of the strain and benefit the exploitation of cyanothece sp. 113.
Keywords:Cyanothece sp  113  growth  exopolysaccharide production
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