| 海洋细菌HW08的鉴定及其所产低温碱性蛋白酶Ps5的分离纯化 |
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| 引用本文： | 杨成业,郝建华,孙谧.海洋细菌HW08的鉴定及其所产低温碱性蛋白酶Ps5的分离纯化[J].海洋湖沼通报,2011(2):107-114. |
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| 作者姓名： | 杨成业 郝建华 孙谧 |
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| 作者单位： | 1. 中国海洋大学医药学院,山东青岛,266003;黄海水产研究所海洋产物资源与酶工程实验室,山东青岛,266071
2. 黄海水产研究所海洋产物资源与酶工程实验室,山东青岛,266071 |
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| 摘 要： | 由黄海底泥分离得到1株能够稳定高产蛋白酶的菌株,命名为HW08.对该菌株进行形态特征、生理生化分析表明,其为革兰氏阴性杆菌,极生单鞭毛,非发酵型,严格需氧,细胞内不积累多羟基丁酸作为储藏碳源,氧化酶阴性.将该菌16S rDNA序列(GenBank登录号FJ999660)和假单胞菌属中模式菌株16S rDNA序列进行比对...
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| 关 键 词： | 蛋白酶 海洋细菌 鉴定 Pseudomonas lundensis 分离纯化 |
IDENTIFICATION OF BACTERIUM HW08 AND PURIFICATION OF ITS EXTRACELLULAR ALKALINE COLD-ADAPTED PROTEASE PS5 |
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| YANG Chengye,HAO Jianhua,SUN Mi.IDENTIFICATION OF BACTERIUM HW08 AND PURIFICATION OF ITS EXTRACELLULAR ALKALINE COLD-ADAPTED PROTEASE PS5[J].Transaction of Oceanology and Limnology,2011(2):107-114. |
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| Authors: | YANG Chengye HAO Jianhua SUN Mi |
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| Institution: | YANG Chengye~(1,2),HAO Jianhua~2,and SUN Mi~(2*) (1.School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China,2.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China) |
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| Abstract: | A strain named HW08,secreted protease abundantly and steadily,was isolated from Yellow Sea sediment.HW08 is an aerobic bacterium,non-fermentative,gram negative, rods and polar-flagellated.It accumulated Poly-P-hydroxybutyrate as a carbon reserve material and had no oxidase activity.The phylogenetic tree was built using Neighbor-joining method after comparing the 16S rDNA of HW08(Gen Bank accession number FJ999660) with other Pseudomonas spp.type strains.Considering the phenotypical characteristics as well,i... |
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| Keywords: | protease marine bacteria identification Pseudomonas lundensis purification |
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