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| 皱纹盘鲍肝、肾表达序列标签及神经肽Y(NPY)受体基因的克隆与分析 | |
| 引用本文： | 郑明刚,毕元兑,王玲,孙修勤,刘峰,王波.皱纹盘鲍肝、肾表达序列标签及神经肽Y(NPY)受体基因的克隆与分析[J].海洋湖沼通报,2011(1):94-100. |
| 作者姓名： | 郑明刚 毕元兑 王玲 孙修勤 刘峰 王波 |
| 作者单位： | 1. 国家海洋局第一海洋研究所海洋生态中心,山东,青岛,266061 2. 青岛经济技术开发区卫生局卫生监督所,山东,青岛,266555 3. 青岛大学化学化工与环境学院,山东,青岛,266071 |
| 基金项目： | 基于生态系统的典趔海域生物资源综合修复与调控技术研究及示范，海洋-所基本科研业务专项 |
| 摘 要： | 通过构建皱纹盘鲍(Haliotis discus Hannai)cDNA文库并进行大规模测序,获得了1282条高质量的ESTs序列,对这些序列进行拼接,获得了包括650条序列在内的244组片段重叠群(Contigs)和632个单一序列(Singletons).使用BlastX软件对876个Unigenes(Uniger... |
| 关 键 词： | 皱纹盘鲍 生物信息学 表达序列标签 神经肽Y受体 |
PRELIMINARY ANALYSIS OF ESTS(EXPRESSED SEQUENCE TAGS) FROM LIVE AND KINEY OF HALIOTIS DISCUS HANNAI AND CLONING OF NPY (NEUROPEPTIDE Y) RECEPTOR GENE |
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| ZHENG Minggang,BI Yuandui,WANG Ling,SUN Xiuqin,LIU Feng,WANG Bo.PRELIMINARY ANALYSIS OF ESTS(EXPRESSED SEQUENCE TAGS) FROM LIVE AND KINEY OF HALIOTIS DISCUS HANNAI AND CLONING OF NPY (NEUROPEPTIDE Y) RECEPTOR GENE[J].Transaction of Oceanology and Limnology,2011(1):94-100. | |
| Authors: | ZHENG Minggang BI Yuandui WANG Ling SUN Xiuqin LIU Feng WANG Bo |
| Institution: | ZHENG Minggang1,BI Yuandui2,WANG Ling3,SUN Xiuqin1,LIU Feng,and WANG Bo1(1.First Institute of Oceanography,Qingdao 266101,China,2.Institute of Health Supervision of Qingdao Economic and TechnologicalDevelopment Area,Qingdao 266555,3.College of Chemistry and ChemicalEngineering and Environment,Qingdao University,Qingdao 266071,China) |
| Abstract: | A cDNA library was constructed from liver and kidney of abalone(Haliotis discus Hannai) and 1445 clones were selected for sequencing.One thousand and two hundred eighty two high qualitative ESTs were obtained through bioinformatics analyses.Splicing of these sequences,244 contigs(consisting of 650 sequences) and 632 singletons were obtained.Using BlastX program for the Homologous comparison,393 unigenes shared significant homology with known sequences in protein database of NCBI accounted for 45% of the num... |
| Keywords: | Haliotis discus Hannai bioinformatics express sequence tag neuropeptide Y |
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