| 日本沼虾黑鳃病几种同工酶的变化与病理分析 |
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| 引用本文： | 吴惠仙,薛俊增.日本沼虾黑鳃病几种同工酶的变化与病理分析[J].海洋湖沼通报,2002(1):32-37. |
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| 作者姓名： | 吴惠仙 薛俊增 |
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| 作者单位： | 1. 浙江大学生命科学学院,杭州,310012
2. 杭州师范学院生物系,杭州,310036 |
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| 基金项目： | 杭州市科技计划项目(99121B07) |
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| 摘 要： | 酯酶（EST），过氧化物酶（POD），乳酸脱氢酶（LDH）和苹果酸脱氢酶（MDH）同工酶在日本沼虾中存在着组织和器官的特异性，患黑鳃病后酶带发生显著变化。在肌肉、肝脏、心脏和鳃中病虾较健康虾的LDH酶谱分别缺失了2、6、2和1条酶带，同时肝脏中新增了1条酶带；POD酶谱分别缺失了0、7、4和2酶带，肌肉中新增了2条酶带；EST酶谱分别缺失了0、2、3和1条酶带，肌肉、心脏和鳃中分别新增了1、2和2条酶带；MDH酶谱分别缺失了0、2、1和1条酶带，肌肉和脏脏中各新增了1条酶带。
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| 关 键 词： | 日本沼虾 黑鳃病 工同酶 聚丙烯酰胺凝胶电泳 过氧化物酶 乳酸脱氢酶 |
| 文章编号： | 1003-6482(2002)01-0032-06 |
| 修稿时间： | 2001年4月10日 |
VARIATION OF SEVERAL ISOZYMES IN BLACK BRANCHIAE DISEASE SHRIMP,MACROBRACHIUM NIPPONENSE ,AND A PRELIMINARY PATHOLOGYLICAL ANALYSIS |
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| WU Huixian.VARIATION OF SEVERAL ISOZYMES IN BLACK BRANCHIAE DISEASE SHRIMP,MACROBRACHIUM NIPPONENSE ,AND A PRELIMINARY PATHOLOGYLICAL ANALYSIS[J].Transaction of Oceanology and Limnology,2002(1):32-37. |
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| Authors: | WU Huixian |
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| Abstract: | The four isozymes (esterase, peroxidase, lactate dehydrogenase and malate dehydrogenase) varied in the muscle, hepatopancreas, heart and branchiae of the fresh water shrimp, macrobrachium nipponense. The electrophoretogram changed greatly in the black branchiae disease shrimp compared with the health shrimp. The LDH disappeared 2 bands, 6 bands, 2 bands and 1 band in muscle, hepatopancreas, heart and branchiae respectively and appeared 1 new band in hepatopancreas of the disease shrimp. The POD disappeared 7 bands, 4 bands and 2 bands in hepatopancreas, heart and branchiae respectively and appeared 2 new bands in muscle of the disease shrimp. The EST disappeared 2 bands, 3 bands and 1 band in hepatopancreas, heart and branchiae and appeared 1 new band, 2 new bands and 2 new bands in muscle, heart and branchiae of the disease shrimp respectively. The MDH disappeared 2 bands, 1 band and 1 band in hepatopancreas, heart and branchiae and appeared 1 new band and 1 new band in muscle and hepatopancreas of the disease shrimp respectively. |
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| Keywords: | Macrobrachium nipponense black branchiae disease Isozyme polyacrylamide gel electrophoresis |
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