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栉孔扇贝(Chlamys farreri)受精过程的细胞学观察
引用本文:任素莲,王德秀,绳秀珍,王如才,高东阳.栉孔扇贝(Chlamys farreri)受精过程的细胞学观察[J].海洋湖沼通报,2000(1):24-29.
作者姓名:任素莲  王德秀  绳秀珍  王如才  高东阳
作者单位:青岛海洋大学水产学院养殖系,青岛,266003
基金项目:国家攀登计划,PD-B6-3-1,
摘    要:用铁-苏木精染色、整体封片的方法对栉孔扇贝受精过程进行了细胞学观察。受精前,卵子处于第一次成熟分裂中期。精子入卵后,成熟分裂重新启动,过程如下:⑴精核去致密,体积迅速增大,卵子染色体变粗。⑵第一次成熟分裂结束,放出第一极体,精核扩散速度减慢。⑶卵子发育到第二次成熟分裂中期,精核膨胀至至最大体积,然后开台弥散。⑷第二次成熟分裂结束,雄原核出现。⑸雌原核出现。⑹雌、雄原核以质相互融合,然后凝缩形成染色

关 键 词:栉孔扇贝  减数分裂  受精  细胞学观察
文章编号:1003-6482(2000)01-0024-06

CYTOLOGICAL OBESERVATION ON FERTILIZATION OF CHLAMYS FARRERI
REN Sulian,WANG Dexiu,SHEN Xiuzheng,WANG Rucai,GAO Dongyang.CYTOLOGICAL OBESERVATION ON FERTILIZATION OF CHLAMYS FARRERI[J].Transaction of Oceanology and Limnology,2000(1):24-29.
Authors:REN Sulian  WANG Dexiu  SHEN Xiuzheng  WANG Rucai  GAO Dongyang
Institution:REN Sulian ,WANG Dexiu ,SHEN Xiuzheng ,WANG Rucai ,GAO Dongyang (Aquaculture departxnent, Fishery college, ocean university of qingdao, qingdao, 266003)
Abstract:Meiotic maturation and pronucleus form in fertilized eggs of chlamys farieri were studied by optical microscope. The eggs were at metaprophase of first meiosis before they were fertilized. The .maturation division,has, begun after sperm. The progress is as follows: (1) The sperm nucleus has a rapid dispersion. (2) The sperm nucleus dispersion slows down with the completion of first meiosis. (3) The sperm nucleus teaches its maximum size at metaphase of the second meiosis. (4) The male pronucleus forms when the second meiosis is completed. (5) The female pronucleus forms. (6) The male pronucleus and female pronucleus combined and their chromo- somes combined with each other and form a zygote nucleus finally. Then the chro- mosome condenses and ranges on their metaphase plate of first cleavage.. Polysperm and polyronucleus were obeserved and the polypronucleus can comb- ine with the female pronucleus and form polyspindles.
Keywords:chlamys farrieri  fertilization  meiosis  cytological obesevation
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