| 扩增仿刺参SSR和ISSR指纹技术的初步研究 |
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| 引用本文： | 闫晗,侯林,毕相东,王雪.扩增仿刺参SSR和ISSR指纹技术的初步研究[J].海洋湖沼通报,2006(1):68-74. |
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| 作者姓名： | 闫晗 侯林 毕相东 王雪 |
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| 作者单位： | 辽宁师范大学,辽宁,大连,116029 |
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| 基金项目： | 国家973计划项目(G1999012005) |
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| 摘 要： | 利用SSR(Simple Sequence Repeats)技术和ISSR(Inter Simple Sequence Repeats)技术对仿刺参(Apostichopus Japonicus)进行了PCR扩增。探索了刺参基因组DNA的提取方法。为了得到更好的PCR扩增结果,对引物浓度、Mg2 浓度、dNTP浓度、模板浓度、Taq浓度和退火温度及循环数等方面进行了研究,从而摸索出一个适合刺参SSR和ISSR分析的反应体系和反应条件,并对PCR反应中的一些特定影响因子进行了分析。
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| 关 键 词： | 仿刺参 SSR ISSR 条件优化 |
| 文章编号： | 1003-6482（2006）01-0068-07 |
| 收稿时间： | 2005-06-27 |
| 修稿时间： | 2005年6月27日 |
PRELIMINARY STUDY ON FINGERPRINT ON SSR AND ISSR OF APOSTICHOPUS JAPONICUS |
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| YAN Han,HOU Lin,BI Xiangdong,WANG Xue.PRELIMINARY STUDY ON FINGERPRINT ON SSR AND ISSR OF APOSTICHOPUS JAPONICUS[J].Transaction of Oceanology and Limnology,2006(1):68-74. |
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| Authors: | YAN Han HOU Lin BI Xiangdong WANG Xue |
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| Institution: | College of Life Science Liaoning Normal University,Dalian 116029,China |
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| Abstract: | SSR technique and ISSR technique were applied to amplifying the genomic DNA of S.Japonicus.We studied a suitable method for DNA extraction.In order to produce clear amplified fragments,the conditions for SSR and ISSR analysis of S.Japonicus were optimized,such as the concentrations of primer,Mg~(2 ),dNTP,DNA and Taq.The reaction systems and reaction conditions suitable for SSR and ISSR analysis were obtained.And the influential factors in the Polymerase Chain Reaction were analyzed. |
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| Keywords: | Apostichopus Japonicus SSR ISSR optimization |
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