| 淇河鲫的RAPD标记及遗传多样性 |
| |
| 引用本文： | 冯建新,张西瑞,周哓林,陈杰,王立海.淇河鲫的RAPD标记及遗传多样性[J].海洋湖沼通报,2003(4):90-94. |
| |
| 作者姓名： | 冯建新 张西瑞 周哓林 陈杰 王立海 |
| |
| 作者单位： | 河南省水产科学研究所,郑州,450044 |
| |
| 基金项目： | 河南省自然科学基金 (项目编号 :0 2 1 1 0 31 5 0 0 )资助项目 |
| |
| 摘 要： | 利用40条随机引物对淇河鲫种群进行了RAPD扫描，并与彭泽鲫、普通野鲫鱼进行了比较；共有31条引物有扩增产物，扩增的DNA片段长度在250～1000bp之间；引物S42、S50和S184对三种群的扩增产物有显著差异，可作为其分子遗传标记。用10条引物对10尾淇河鲫鱼的遗传多样性进行了分析，其平均遗传相似率为0．782。
|
| 关 键 词： | 遗传多样性 RAPD标记 随机引物 扩增产物 彭泽鲫 种群 淇河鲫鱼 分子遗传标记 DNA片段 |
| 文章编号： | 1003-6482(2003)04-0090-05 |
| 修稿时间： | 2003年2月25日 |
RAPD MARKERS AND GENETIC DIVERSITY OF CARASSIUS AURATUS IN THE QIHE RIVER |
| |
| FENG Jianxin,ZHANG Xirui,ZHOU Xiaolin,CHEN Jie WANG Lihai.RAPD MARKERS AND GENETIC DIVERSITY OF CARASSIUS AURATUS IN THE QIHE RIVER[J].Transaction of Oceanology and Limnology,2003(4):90-94. |
| |
| Authors: | FENG Jianxin ZHANG Xirui ZHOU Xiaolin CHEN Jie WANG Lihai |
| |
| Abstract: | Carassius auratus in the Qihe River were analyzed by the RAPD method using 40 primers and compared with Pengze Crucian Carp and common Crucian Carp. The experiment shows that 31 primers were effective. The length of the fragment was 250bp 1000bp. Among the three populations, the differences of fingerprints obtained by primers S42,S50,S184 were so obvious that they might be used as molecular markers.The genetic diversity of 10 Carassius auratus in the Qihe River was studied with 10 primers. The average genetic similarity was 78.2%. |
| |
| Keywords: | Carassius auratus in Qihe River genetic diversity RAPD markers |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
