| 海洋微藻多糖提取纯化条件的研究 |
| |
| 引用本文： | 吴曼,李文权,张赛金,陈清花.海洋微藻多糖提取纯化条件的研究[J].海洋技术,2004,23(1):9-12. |
| |
| 作者姓名： | 吴曼 李文权 张赛金 陈清花 |
| |
| 作者单位： | 厦门大学海洋学系、亚热带海洋研究所,福建,厦门,361005 |
| |
| 基金项目： | 国家自然科学基金资助项目 ( 39870 56 5) |
| |
| 摘 要： | 研究了提取介质、超声条件和洗脱条件对海洋微藻多糖提取纯化效果的影响。实验结果表明不同pH的提取介质可以提取得到不同种类的多糖，超声条件以300W、20min最佳，用葡聚糖凝胶Sephadex-G200柱(长50cm，直径2cm)纯化粗多糖，当加样量为4ml，用0．2mol／L NaCl溶液洗脱效果最好。
|
| 关 键 词： | 海洋微藻 多糖 提取纯化 洗脱 |
| 文章编号： | 1003-2029(2004)01-0009-04 |
Study on the Conditions of Extracting and Purifying of Polysaccharide from Marine Microalgae |
| |
| WU Man,LI Wen-quan,ZHANG Sai-jin,CHEN Qing-hua.Study on the Conditions of Extracting and Purifying of Polysaccharide from Marine Microalgae[J].Ocean Technology,2004,23(1):9-12. |
| |
| Authors: | WU Man LI Wen-quan ZHANG Sai-jin CHEN Qing-hua |
| |
| Abstract: | The paper investigates the effect of extracting medium, ultrasonic con dition and eluting condition on extraction and purification of polysaccharides f rom marine microalgae. The experimental results indicate that the resulting poly saccharides from marine microalgae are distinct as varied pH in medium. The opti mal ultrasonic power is 300W and the optimal ultrasonic duration is 20min. When the coarse polysaccharides are purified through the Sephadex-G200 column (lengt h: 50cm, diameter: 2cm),the optimal volume of sample added into the column is 4mL, and the optimal eluant is 0 2 mol/L NaCl. |
| |
| Keywords: | marine microalgae polysaccharide extraction and purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
