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| 超声波萃取-高效液相色谱法在海洋沉积物样品多环芳烃分析中的运用 | |
| 引用本文： | 黄宏,黄长江,陈图锋,朱爱意.超声波萃取-高效液相色谱法在海洋沉积物样品多环芳烃分析中的运用[J].海洋技术,2006,25(1):67-72. |
| 作者姓名： | 黄宏 黄长江 陈图锋 朱爱意 |
| 作者单位： | 1. 汕头大学海洋生物研究所,广东,汕头,515063 2. 汕头大学海洋生物研究所,广东,汕头,515063;温州医学院海洋科学研究所,浙江,温州,325000 3. 汕头大学分析测试中心,广东,汕头,515063 4. 浙江海洋学院海洋科学与技术学院,浙江,舟山,316042 |
| 基金项目： | 国家自然科学基金资助项目(40176029)，广东省重大科技兴海项目(A20000502F02) |
| 摘 要： | 建立用超声波萃取-高效液相色谱法同时检测海洋沉积物中16种优先监控的多环芳烃(PAH s)的方法。本方法采用丙酮作为萃取溶剂,在超声波作用下萃取出泥样中的PAH s,萃取液经浓缩后直接进行高效液相色谱分析。经对色谱条件和萃取条件进行优化后,各PAH组分的线性范围为1~500 ng之间,相关系数0.9921~0.999,检出限0.1~4.6 ng,达到海洋沉积物PAH s的检测要求。运用本法对粤东大规模增养殖区柘林湾表层沉积物PAH s进行检测,各PAH组分加标回收率为69.2%~108%,总平均达89.2%,相对标准偏差为0.5%~10.3%。本方法只需要萃取、浓缩和定容三个步骤,具有简便、迅速、高效、分析成本低的特点。 |
| 关 键 词： | 多环芳烃(PAHs) 海洋沉积物样品 超声波萃取 高效液相色谱(HPLC) |
| 文章编号： | 1003-2029（2006）01-0067-06 |
| 收稿时间： | 2005-05-20 |
| 修稿时间： | 2005年5月20日 |
Determination of PAHs of Marine Sediments by HPLC Following Ultrasonic Extraction |
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| HUANG Hong,HUANG Chang-jiang,CHEN Tu-fong,ZHU Ai-yi.Determination of PAHs of Marine Sediments by HPLC Following Ultrasonic Extraction[J].Ocean Technology,2006,25(1):67-72. | |
| Authors: | HUANG Hong HUANG Chang-jiang CHEN Tu-fong ZHU Ai-yi |
| Institution: | 1. Institute of Marine Biology, Shantou University, Shantou 515063; 2, Analysis Center, Shantou University, Shantou 515063, China 3, School of Marine Science and Technique, Zhejiang Ocean University, Zhoushan 31600 |
| Abstract: | A method using ultrasonic extraction combined with separation by HPLC was established for the simultaneous determination of 16 PAHs in marine sediments.The PAHs(Polycyclic Aromatic Hydrocarbons) in the sediments were extracted by acetone in an ultrasonic bath and directly determinated by high-performance liquid chromatography after concentrated,in which the clean-up step is not necessary.After the chromatographic conditions and ultrasonic extraction were optimized,for each PAH component the linear limit ranges from 1 to 500 ng,the cofficient of correlation ranges from 0.9921 to 0.9999,and the detection limit ranges from 0.1 to 4.6 ng,which are up to scratch for marine sediment.Using the established method to measure the PAHs of sediments from Zhelin Bay of Eastern Guangdong with large-scale mariculture,the recoveries of PAHs ranged from 69.2% to 108% with a total average of 89.2% and standard deviations from 0.5 to10.3.The method is simple,fast,highly active,and low cost. |
| Keywords: | polycyclic aromatic hydrocarbons marine sediment samples uitrasonic extraction high-performance liquid chromatography |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |