Short sequence effect of ancient DNA on mammoth phylogenetic analyses |
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| Authors: | Guilian Sheng Lianjuan Wu Xindong Hou Junxia Yuan Shenghong Cheng Bojian Zhong and Xulong Lai |
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| Institution: | (1) Key Laboratory of Biogeology and Environmental Geology of Ministry of Education, China University of Geosciences, Wuhan, 430074, China;(2) Institute of Ecology & Environment, China University of Geosciences, Wuhan, 430074, China |
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| Abstract: | The evolution of Elephantidae has been intensively studied in the past few years, especially after 2006. The molecular approaches
have made great contribution to the assumption that the extinct woolly mammoth has a close relationship with the Asian elephant
instead of the African elephant. In this study, partial ancient DNA sequences of cytochrome b (cyt b) gene in mitochondrial genome were successfully retrieved from Late Pleistocene Mammuthus primigenius bones collected from Heilongjiang Province in Northeast China. Both the partial and complete homologous cyt b gene sequences and the whole mitochondrial genome sequences extracted from GenBank were aligned and used as datasets for
phylogenetic analyses. All of the phylogenetic trees, based on either the partial or the complete cyt b gene, reject the relationship constructed by the whole mitochondrial genome, showing the occurrence of an effect of sequence
length of cyt b gene on mammoth phylogenetic analyses. |
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| Keywords: | Mammuthus primigenius ancient mitochondrial DNA cytochrome b (cyt b) gene phylogenetic analysis |
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