| 太湖沉积物中微生物多样性垂向分布特征 |
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| 引用本文： | 赵兴青,杨柳燕,尹大强,王汝成,陆现彩,陆建军.太湖沉积物中微生物多样性垂向分布特征[J].地学前缘,2008,15(6). |
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| 作者姓名： | 赵兴青 杨柳燕 尹大强 王汝成 陆现彩 陆建军 |
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| 基金项目： | 国家重点基础研究发展计划"973"项目
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江苏省自然科学基金项目
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| 摘 要： | 运用化学分析方法和PCR-DGGE技术,从沉积物化学及分子生物学角度对太湖沉积物理化性质(pH、Eh)、营养盐及微生物多样性的垂向分布及相关性进行研究。结果表明:沉积物-上覆水接触界面处于轻度还原状态,在表层8.0cm左右以下,Eh随沉积深度的增加迅速下降,还原性逐渐增强,到15.0～22.5cm深度区间内,Eh值基本稳定,还原性最强,之后随深度的进一步增加,Eh呈"之"型缓慢升高。沉积物pH随深度的增加先降低后缓慢升高,pH在整个剖面上变化幅度不大,在7.2～7.6变动。沉积物中含有丰富的营养盐,总氮(TN)、总磷(TP)最高含量分数和有机质(OM)的最高百分比分别为2.436mg/g、0.731mg/g和3.817%,其剖面特征表明,沉积物表层TN和OM远高于底层,其含量随深度增加而降低。TP随着沉积深度的增加呈"之"型缓慢减少。不同深度沉积物的微生物群落呈现出明显的空间分布多样性差异,不同深度沉积物的微生物群落结构之间的相似性和动态性存在差异。多元相关分析结果显示,TN与OM显著相关,理化指标、营养盐中任一指标含量与微生物群落多样性指标之间存在相关性,但不显著,微生物多样性是营养盐及环境物理、化学和生物等多方面共同作用的结果。
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| 关 键 词： | 微生物多样性 沉积物 垂向分布 变性梯度凝胶电泳(DGGE) 16S rDNA |
Vertical distrbution of the microbial community in modern lacustrine sedi-ments of Taihu Luae |
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| Zhao Xingqing,Yang Liuyan,Yin Daqiang,Wang Rucheng,Lu Xiancai,Lu Jianjun.Vertical distrbution of the microbial community in modern lacustrine sedi-ments of Taihu Luae[J].Earth Science Frontiers,2008,15(6). |
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| Authors: | Zhao Xingqing Yang Liuyan Yin Daqiang Wang Rucheng Lu Xiancai Lu Jianjun |
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| Abstract: | The paper studies the physicochemical characteristics,nutritions and the microbial community diversity of sediment core samples in the Taihu Lake with fumigation-digestion and PCR-DGGE analysis.The results indicated that the water-sediment interface was in a state of mild reduction.Below the surface of sediments,Eh decreased rapidly with the increase in depth,and it is under the anaerobic condition except the top 8.0 cm layer of the sediment;in the depth ranging from 15.0 to 22.5 cm,the Eh value is basically stable.Further increasing the depth of sediment layers,the Eh value increased in the "z" style.The pH value decreased first and then slowly increased with the increase in the depth of sediment layers;the pH value changed slightly in the profile of sediments and ranged from 7.2 to 7.6.The TN,TP and OM concentrations in sediments are high,and their maximum concentrations reached 2.436 mg/g,0.731 mg/g and 3.817%,respectively.Their vertical profiles showed that the TN and OM concentrations decreased with the increase in the depth of sediment layers.The TP concentration slowly decreased with the increase in the depth of sediment layers in the "z" style.There are prominent differences in microbial diversity spatially;the comparability and the dynamic characteristics of community structures between different sediment samples at different depth are different.In order to test the correlation of the parameters,the properties of the sediment samples were analyzed by correlating all the depth of the samples with pH,Eh,OM,TN,TP and the Shannon-Weaver index(H').The little significant correlation at the α = 0.05 level was observed between the environmental parameters.However,the TN concentration and the percentage of organic matter(OM)are significantly correlated.The microbial diversity is jointly affected by the nutrient,the physical,chemical and biological characteristics,etc. |
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| Keywords: | 16SrDNA |
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