| 冀中地热区深部热水微生物群落组成及其功能预测 |
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| 引用本文： | 赵佳怡,甄世军,张翠云,殷密英,张胜.冀中地热区深部热水微生物群落组成及其功能预测[J].地球学报,2021,42(5):605-616. |
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| 作者姓名： | 赵佳怡 甄世军 张翠云 殷密英 张胜 |
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| 作者单位： | 中国地质科学院水文地质环境地质研究所;中国地质调查局/河北省地下水污染机理与修复重点实验室 |
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| 基金项目： | 中国地质调查局地质调查项目(编号: DD20189112);中国地质科学院水文地质环境地质研究所基本科研业务费专项(编号: SK202010) |
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| 摘 要： | 了解深部微生物群落组成和功能对探索早期生命起源和利用其独特功能均有重要意义.地热区深部碳酸盐岩岩溶-裂隙热储环境中微生物群落组成和功能特性仍然不清.为了探测冀中地热区深部热水微生物群落组成与功能特性,以浅层水和土壤为参照,利用冀中地热区地热科学钻探孔抽水试验,采集深部热水样,同时采集每个钻孔上方附近的大田农灌井浅层水和土壤样品,用于16S rRNA基因高通量测序,并使用PICRUSt软件进行功能预测.结果显示冀中地热区深部热水含有38个菌门,541个菌属,以细菌为主(97.5％),古菌很少(2.5％),特征性菌群主要是厚壁菌门Firmicutes、硝化螺旋菌门Nitrospirae、热袍菌门Thermotogae和广古菌门Euryarchaeota,优势菌属包括硫酸盐还原菌,固碳作用、发酵作用和硫酸盐还原作用可能较强,而产甲烷作用和反硝化作用可能很弱.深部热水出现好氧嗜温菌,说明在大流量抽水条件下,深部热水受到富含变形菌门Proteobacteria浅层水的补给.本次研究揭示了冀中地热区深部碳酸盐岩岩溶-裂隙热储层含有种类丰富、功能多样的微生物群落,微生物群落特征可作为深部热水与浅层水水力联系的指示剂.
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| 关 键 词： | 微生物群落特征 功能特性 深部热水 地热科学钻探 冀中地热区 |
Composition and Functional Prediction of Microbial Communities in Deep Geothermal Water from Jizhong (Central Hebei) Geothermal Area |
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| ZHAO Jia-yi,ZHEN Shi-jun,ZHANG Cui-yun,YIN Mi-ying,ZHANG Sheng.Composition and Functional Prediction of Microbial Communities in Deep Geothermal Water from Jizhong (Central Hebei) Geothermal Area[J].Acta Geoscientia Sinica,2021,42(5):605-616. |
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| Authors: | ZHAO Jia-yi ZHEN Shi-jun ZHANG Cui-yun YIN Mi-ying ZHANG Sheng |
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| Institution: | Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences;Key Laboratory of Groundwater Remediation of Hebei Province and China Geological Survey |
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| Abstract: | The understanding of the composition and functions of deep microbial communities is of great significance for exploring the origin of early lives and utilizing their unique functions. The composition and functions of microbial communities in deep carbonate karst-fissure geothermal storage environment remain unclear. In order to explore the composition and prediction of the functions of microbial communities in the deep geothermal water from the deep carbonate rock karst-fissure geothermal storage environment, the authors, taking shallow water and soil as the references, collected deep geothermal water samples by the pumping tests using the geothermal scientific drilling holes located in Jizhong geothermal area and also collected shallow water samples from farm irrigation wells and soil samples near the top of each drill hole, respectively for 16S rRNA gene high-throughput sequencing and functional predictions using PICRUSt. The results show that there exist 38 phyla and 541 genera in the deep geothermal water of Jizhong geothermal area, of which bacteria are dominant (97.5%), whereas archaea are rare (2.5%), and characteristic microbial communities comprise mainly Firmicutes, Nitrospirae, Thermotogae and Euryarchaeota. The dominant bacterial genera include sulfate-reducing bacteria. Carbon fixation, fermentation and sulfate reduction might be relatively strong, whereas methanogenesis and denitrification might be weak in the deep geothermal water. Aerobic, mesophilic bacteria occurring in the deep geothermal water indicate that the deep geothermal water is recharged by the shallow water rich in proteobacteria under the condition of large flow pumping. This study has revealed that the deep carbonate rock karst-fracture geothermal reservoirs contain abundant microbial communities with various functions in Jizhong geothermal area, and that the characteristics of microbial communities can be used as indicators of the hydraulic connection between deep geothermal water and shallow water. |
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| Keywords: | microbial community characteristics functional characteristics deep geothermal water geothermal scientific drilling Jizhong geothermal area |
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