| 地下水硝酸盐氮同位素分析最新方法——细菌反硝化法 |
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| 引用本文： | 毛绪美,罗泽娇,李永勇,刘存富.地下水硝酸盐氮同位素分析最新方法——细菌反硝化法[J].地球学报,2005,26(Z1):44-47. |
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| 作者姓名： | 毛绪美 罗泽娇 李永勇 刘存富 |
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| 作者单位： | 中国地质大学环境学院，湖北武汉，430074;中国地质大学环境学院，湖北武汉，430074;中国地质大学环境学院，湖北武汉，430074;中国地质大学环境学院，湖北武汉，430074 |
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| 基金项目： | 本文由国家白然科学基金青年基金项目“浅层地下水耗氧反硝化脱氮机理及15N,18O标记研究”（批准号40402020)资助 |
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| 摘 要： | 细菌反硝化法是目前地下水中硝酸盐氮同位素分析的最新方法，包括反硝化菌的选取与培养、反硝化菌将硝酸盐完全转化成N2O气体、N2O气体的提取与纯化、N2O气体氮同位素测定。与传统方法相比，细菌反硝化法可分析低浓度微量水中硝酸盐氮同位素，且更为快捷可靠。
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| 关 键 词： | 氮同位素 细菌反硝化 硝酸盐 地下水 |
Bacterial Denitrification: A New Method for Nitrogen Isotopic Analysis of Nitrate in Groundwater |
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| MAO Xume,LUO Zejiao,LI Yongyong and LIU Cunfu.Bacterial Denitrification: A New Method for Nitrogen Isotopic Analysis of Nitrate in Groundwater[J].Acta Geoscientia Sinica,2005,26(Z1):44-47. |
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| Authors: | MAO Xume LUO Zejiao LI Yongyong and LIU Cunfu |
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| Abstract: | Bacterial denitrification is a new method for nitrogen isotopic analysis of nitrate in groundwater. Its process includes selection and cultivation of denitrifying bacteria strains, complete conversion of sample nitrate to nitrous oxide by denitrifying bacteria, extraction and purification of nitrous oxide, and isotopic analysis of nitrous oxide. Compared with the combustion method, the bacterial denitrification method can analyze nitrogen isotope of nitrate in trace amounts of water samples with lower concentration, and is hence more rapid, convenient and reliable. |
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