| 短小芽孢杆菌-蒙脱石相互作用实验研究 |
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| 引用本文： | 赵玉连,代群威,董发勤,许凤琴,邬琴琴,王岩.短小芽孢杆菌-蒙脱石相互作用实验研究[J].岩石矿物学杂志,2015,34(6):939-944. |
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| 作者姓名： | 赵玉连 代群威 董发勤 许凤琴 邬琴琴 王岩 |
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| 作者单位： | 西南科技大学环境与资源学院, 四川 绵阳 621010;西南科技大学环境与资源学院, 四川 绵阳 621010;固体废物处理与资源化省部共建教育重点实验室, 四川 绵阳 621010;固体废物处理与资源化省部共建教育重点实验室, 四川 绵阳 621010;西南科技大学环境与资源学院, 四川 绵阳 621010;西南科技大学环境与资源学院, 四川 绵阳 621010;西南科技大学环境与资源学院, 四川 绵阳 621010 |
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| 基金项目： | 国家自然科学基金重点项目(41130746);国家自然科学基金青年项目(41102212);西南科技大学研究生创新基金项目(14ycxjj0033) |
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| 摘 要： | 主要探讨短小芽孢杆菌与钙基蒙脱石相互作用过程中的现象及机理。SEM结果显示,大量土壤菌黏附、包裹于蒙脱石表面。加入蒙脱石后,短小芽孢杆菌-钙基蒙脱体系中有机酸含量明显降低。XRD结果表明,短小芽孢杆菌作用后蒙脱石层间距增大,001面衍射峰钝化。FTIR显示作用后蒙脱石出现了来自于土壤菌的新峰,其他基团峰出现蓝移或红移现象。经二阶导数红外光谱分析验证,蒙脱石中确实出现了来自于土壤菌的其他基团振动,揭示土壤菌代谢产物进入蒙脱石层间域内的初步可能性,这将为矿物-微生物相互作用间的机理研究提供新依据。
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| 关 键 词： | 蒙脱石 FTIR 二阶导数红外光谱 短小芽孢杆菌 矿物-微生物相互作用 |
| 收稿时间： | 2015/1/20 0:00:00 |
| 修稿时间： | 2015/4/2 0:00:00 |
An experimental study of the interaction between Bacillus pumilus and montmorillonite |
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| ZHAO Yu-lian,DAI Qun-wei,DONG Fa-qin,XU Feng-qin,WU Qin-qin and WANG Yan.An experimental study of the interaction between Bacillus pumilus and montmorillonite[J].Acta Petrologica Et Mineralogica,2015,34(6):939-944. |
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| Authors: | ZHAO Yu-lian DAI Qun-wei DONG Fa-qin XU Feng-qin WU Qin-qin and WANG Yan |
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| Institution: | School of Environment and Resource, Southwest University of Science and Technology, Mianyang 621010, China;School of Environment and Resource, Southwest University of Science and Technology, Mianyang 621010, China;Key Laboratory of Solid Waste Treatment and Resource Recycle(SWUST), Ministry of Education, Mianyang 621010, China;Key Laboratory of Solid Waste Treatment and Resource Recycle(SWUST), Ministry of Education, Mianyang 621010, China;School of Environment and Resource, Southwest University of Science and Technology, Mianyang 621010, China;School of Environment and Resource, Southwest University of Science and Technology, Mianyang 621010, China;School of Environment and Resource, Southwest University of Science and Technology, Mianyang 621010, China |
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| Abstract: | This paper aims to investigate the phenomena and the mechanism of the interaction between Bacillus pumilus and Ca-montmorillonite. SEM results showed that a large number of Bacillus pumilus attached to and wrapped up the surface of Ca-montmorillonite. The content of organic acids of the Bacillus pumilus-Ca-montmorillonite system decreased obviously after joining the Ca-montmorillonite. XRD results showed that the interlayer spacing of Ca-montmorillonite increased, and diffraction peak of 001 exhibited passivation after interacting with Bacillus pumilus. FTIR results showed that peaks assigned to soil bacteria appeared, and peaks of the structural groups of Ca-montmorillonite exhibited blue-shift or red-shift as well. Verification by second derivative FTIR spectroscopy analysis is that Ca-montmorillonite really shows the vibration of other group assigned to soil bacteria. It is thus inferred that there is a preliminary possibility that the metabolic products of soil bacteria enter the interlayer space of Ca-montmorillonite. The result obtained by the authors will provide a new basis for the mechanism of the interaction between mineral and microorganism. |
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| Keywords: | montmorillonite FTIR second derivative FTIR spectroscopy analysis Bacillus pumilus microbe-mineral interaction |
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