| 正常菌与可吸入矿物细颗粒作用致突变性研究 |
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| 引用本文： | 马骥,董发勤,邓建军,曾娅莉.正常菌与可吸入矿物细颗粒作用致突变性研究[J].岩石矿物学杂志,2017,36(6):903-908. |
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| 作者姓名： | 马骥 董发勤 邓建军 曾娅莉 |
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| 作者单位： | 四川绵阳四○四医院检验科, 四川 绵阳 621000,西南科技大学, 四川 绵阳 621010,四川绵阳四○四医院检验科, 四川 绵阳 621000,四川绵阳四○四医院检验科, 四川 绵阳 621000 |
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| 基金项目： | 国家自然科学基金重点项目（41130746）；国家自然科学基金面上项目（41472046） |
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| 摘 要： | 采用Ames试验分别对蒙脱石、石英、方解石、钠长石4种可吸入矿物细颗粒及其与大肠杆菌、表皮葡萄球菌相互作用48 h后的混悬液作为受试物进行检测,分析IMG是否具有致突变性,及其与人体正常细菌群作用后,IMG/正常菌复合体是否有致突变性毒力改变。结果显示石英以及石英/大肠杆菌复合体对TA98、TA100菌株Ames试验呈阳性反应;石英/表皮葡萄球菌复合体对TA98菌株Ames试验呈阳性反应;而蒙脱石、方解石、钠长石纯矿及其与正常菌作用后的复合体Ames试验结果均为阴性。结果表明石英具有直接致突变作用;石英与正常菌菌作用后复合体依然具有致突变性,但石英表现出的碱基置换突变作用在与表皮葡萄球菌作用后消失,其机制有待进一步研究。
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| 关 键 词： | 可吸入矿物细颗粒(IMG) Ames试验 表皮葡萄球菌 大肠杆菌 致突变性 |
| 收稿时间： | 2017/8/30 0:00:00 |
| 修稿时间： | 2017/10/18 0:00:00 |
A study of the mutagenicity of normal bacteria interacted with inhalable mineral granules |
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| MA Ji,DONG Fa-qin,DENG Jian-jun and ZENG Ya-li.A study of the mutagenicity of normal bacteria interacted with inhalable mineral granules[J].Acta Petrologica Et Mineralogica,2017,36(6):903-908. |
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| Authors: | MA Ji DONG Fa-qin DENG Jian-jun and ZENG Ya-li |
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| Institution: | Sichuan Mianyang 404 Hospital, Mianyang 621000, China,Southwest University of Science and Technology, Mianyang 621010, China,Sichuan Mianyang 404 Hospital, Mianyang 621000, China and Sichuan Mianyang 404 Hospital, Mianyang 621000, China |
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| Abstract: | The mutagenicity of inhalable mineral granules (IMG) and interaction with Epidermis staphylococcus, E. coli were studied by Ames test to analyze whether IMG and IMG/body normal flora complex has mutagenicity. The results show that Ames test of the quartz and quartz/E. coli complex on TA98 and TA100 strains assumes positive reaction, Ames test of quartz/epidermis staphylococcus complex on TA98 strains assumes position reaction, whereas Ames test of montmorillonite, calcite, albite and complex from interaction with body normal flora exhibits negative reaction, which suggests that the quartz has direct mutagenic effect, quartz/body normal flora complex still has mutagenicity, but the mechanism of the phenomenon that base replacement mutation effects of quartz disappeared after interaction with Epidermis staphylococcus needs further research. |
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| Keywords: | inhalable mineral granules (IMG) Ames test Epidermis staphylococcus Escherichia coli mutagenicity |
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